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Title: Halothane inhibits the cholinergic-receptor-mediated influx of calcium in primary culture of bovine adrenal medulla cells

Abstract

Adrenal medulla cells are cholinoceptive cells. Stimulation of the acetylcholine receptor causes the influx of Ca to the cells, and Ca acts as the coupler of the stimulus-secretion coupling. In this study, the authors investigated the effects of halothane on the receptor-mediated influx of /sup 45/Ca using cultured bovine adrenal medulla cells. Halothane at clinical concentrations (0.5-2%) inhibited the influx of /sup 45/Ca caused by carbachol, with simultaneous inhibition of catecholamine secretion. The influx of /sup 45/Ca and the secretion of catecholamines caused by K depolarization were inhibited by a large concentration of Mg, which competes with Ca at Ca channels, but not inhibited by halothane. Inhibition of the /sup 45/Ca influx by halothane was not overcome by increase in the carbachol concentration. Inhibition of the /sup 45/Ca influx by halothane was examined in comparison with that caused by a large concentration of Mg by the application of Scatchard analysis as the function of the external Ca concentration. Halothane decreased the maximal influx of /sup 45/Ca without altering the apparent kinetic constant of Ca to Ca channels. On the contrary, a large concentration of Mg increased the apparent kinetic constant without altering the maximal influx of /sup 45/Ca. Based onmore » these findings, the authors suggest that inhibition of the /sup 45/Ca influx by halothane was not due to the direct competitive inhibition of Ca channels, nor to the competitive antagonism of agonist-receptor interaction. As a possibility, halothane seems to inhibit the receptor-mediated activation of Ca channels through the interference of coupling between the receptor and Ca channels.« less

Authors:
; ;
Publication Date:
Research Org.:
Kyushu Univ. School of Medicine, Japan
OSTI Identifier:
5682454
Resource Type:
Journal Article
Journal Name:
Anesthesiology; (United States)
Additional Journal Information:
Journal Volume: 4
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ACETYLCHOLINE; RECEPTORS; ANESTHETICS; BIOLOGICAL EFFECTS; CALCIUM; MEMBRANE TRANSPORT; ADRENAL GLANDS; ANIMAL CELLS; CALCIUM 45; CATECHOLAMINES; CATTLE; MAGNESIUM; POTASSIUM; TRACER TECHNIQUES; ALKALI METALS; ALKALINE EARTH ISOTOPES; ALKALINE EARTH METALS; AMINES; AMMONIUM COMPOUNDS; ANIMALS; AROMATICS; AUTONOMIC NERVOUS SYSTEM AGENTS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CALCIUM ISOTOPES; CENTRAL NERVOUS SYSTEM DEPRESSANTS; DAYS LIVING RADIOISOTOPES; DOMESTIC ANIMALS; DRUGS; ELEMENTS; ENDOCRINE GLANDS; ESTERS; EVEN-ODD NUCLEI; GLANDS; HYDROXY COMPOUNDS; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; MEMBRANE PROTEINS; METALS; NEUROREGULATORS; NUCLEI; ORGANIC COMPOUNDS; ORGANS; PARASYMPATHOMIMETICS; PHENOLS; POLYPHENOLS; PROTEINS; QUATERNARY COMPOUNDS; RADIOISOTOPES; RUMINANTS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Yashima, N, Wada, A, and Izumi, F. Halothane inhibits the cholinergic-receptor-mediated influx of calcium in primary culture of bovine adrenal medulla cells. United States: N. p., 1986. Web. doi:10.1097/00000542-198604000-00009.
Yashima, N, Wada, A, & Izumi, F. Halothane inhibits the cholinergic-receptor-mediated influx of calcium in primary culture of bovine adrenal medulla cells. United States. https://doi.org/10.1097/00000542-198604000-00009
Yashima, N, Wada, A, and Izumi, F. 1986. "Halothane inhibits the cholinergic-receptor-mediated influx of calcium in primary culture of bovine adrenal medulla cells". United States. https://doi.org/10.1097/00000542-198604000-00009.
@article{osti_5682454,
title = {Halothane inhibits the cholinergic-receptor-mediated influx of calcium in primary culture of bovine adrenal medulla cells},
author = {Yashima, N and Wada, A and Izumi, F},
abstractNote = {Adrenal medulla cells are cholinoceptive cells. Stimulation of the acetylcholine receptor causes the influx of Ca to the cells, and Ca acts as the coupler of the stimulus-secretion coupling. In this study, the authors investigated the effects of halothane on the receptor-mediated influx of /sup 45/Ca using cultured bovine adrenal medulla cells. Halothane at clinical concentrations (0.5-2%) inhibited the influx of /sup 45/Ca caused by carbachol, with simultaneous inhibition of catecholamine secretion. The influx of /sup 45/Ca and the secretion of catecholamines caused by K depolarization were inhibited by a large concentration of Mg, which competes with Ca at Ca channels, but not inhibited by halothane. Inhibition of the /sup 45/Ca influx by halothane was not overcome by increase in the carbachol concentration. Inhibition of the /sup 45/Ca influx by halothane was examined in comparison with that caused by a large concentration of Mg by the application of Scatchard analysis as the function of the external Ca concentration. Halothane decreased the maximal influx of /sup 45/Ca without altering the apparent kinetic constant of Ca to Ca channels. On the contrary, a large concentration of Mg increased the apparent kinetic constant without altering the maximal influx of /sup 45/Ca. Based on these findings, the authors suggest that inhibition of the /sup 45/Ca influx by halothane was not due to the direct competitive inhibition of Ca channels, nor to the competitive antagonism of agonist-receptor interaction. As a possibility, halothane seems to inhibit the receptor-mediated activation of Ca channels through the interference of coupling between the receptor and Ca channels.},
doi = {10.1097/00000542-198604000-00009},
url = {https://www.osti.gov/biblio/5682454}, journal = {Anesthesiology; (United States)},
number = ,
volume = 4,
place = {United States},
year = {Tue Apr 01 00:00:00 EST 1986},
month = {Tue Apr 01 00:00:00 EST 1986}
}