Contaminating fibrin in CPD-blood: solubility in plasma and distribution in blood components following separation

Journal Article · Wed Jan 01 00:00:00 EST 1986 · Thromb. Res.; (United States)

DOI:https://doi.org/10.1016/0049-3848(86)90274-4· OSTI ID:5682072

Skjonsberg, O H; Kierulf, P; Gravem, K; Fagerhol, M K; Godal, H C

In order to estimate the solubility of contaminating fibrin in CPD-blood, thrombin induced fibrin polymerzation in CPD-plasma was examined by light scattering and fibrinopeptide A (FPA) determinations. In addition, I-125 fibrin monomer enriched CPD-blood was used to investigate fibrin monomer retention in blood bags and transfusion filters (170 microns) and fibrin distribution in blood components derived from CPD-blood. Initial fibrin polymerization in CPD-blood occurred after conversion of 15 per cent of the fibrinogen to fibrin, implying that substantial amounts of fibrin may be kept solubilized in CPD-blood bags. Only minor amounts of I-125 fibrin monomers were retained in blood bags (2.4 per cent) and in transfusion filters (2.9 per cent) after sham transfusions. After separating I-125-fibrin monomer enriched CPD-blood into its constituent components, the major part of fibrin (75.0 per cent) could be traced in the cryoprecipitate.

• Research Organization: Univ. of Oslo, Norway
• OSTI ID: 5682072
• Journal Information: Thromb. Res.; (United States), Vol. 1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
FIBRIN
POLYMERIZATION
SOLUBILITY
ANTICOAGULANTS
CITRATES
FILTRATION
GLUCOSE
IODINE 125
PHOSPHATES
POLYMERS
RETENTION
THROMBIN
TISSUE DISTRIBUTION
TRACER TECHNIQUES
TRANSFUSIONS
ALDEHYDES
BETA DECAY RADIOISOTOPES
CARBOHYDRATES
CARBOXYLIC ACID SALTS
CHEMICAL REACTIONS
COAGULANTS
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
HEMATOLOGIC AGENTS
HEMOSTATICS
HEXOSES
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MONOSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDE HYDROLASES
PHOSPHORUS COMPOUNDS
PROTEINS
RADIOISOTOPES
SACCHARIDES
SCLEROPROTEINS
SEPARATION PROCESSES
SERINE PROTEINASES
THERAPY
550201* - Biochemistry- Tracer Techniques