Uterine prostaglandin concentrations in sheep during late pregnancy and adrenocorticotropin-induced labor

Journal Article · Sun Nov 01 00:00:00 EST 1981 · Endocrinology; (United States)

DOI:https://doi.org/10.1210/endo-109-5-1533· OSTI ID:5682019

Evans, C A; Kennedy, T G; Patrick, J E; Challis, J R.G.

Concentractions of prostaglandin E (PGE), PGF, and 6-oxo-PGF/sub 1..cap alpha../ (the hydrolysis product of PGI/sub 2/) were measured by RIA in different areas of myometrium, endometrium, cotyledons, amnion, and chorioallantoic membrane taken from chronically catheterized sheep. The animals were 130 days pregnant and had been treated for 70 h with continuous intrafetal infusions of either ACTH-(1-24) or saline. Maternal and fetal plasma samples were collected at 8-h intervals beginning 48 h before the start of infusion, and maternal plasma progesterone, estrone, and estradiol and fetal plasma cortisol concentrations were estimated by RIAs. As suggested by a significant fall in maternal plasma progesterone and rises in maternal plasma estrone and fetal plasma cortisol concentrations, sheep receiving intrafetal ACTH were in early labor at the time tissue samples were taken for PG determinations. For myometrium, analysis of variance indicated a significant effect of uterine area of PG concentrations. Concentrations of 6-oxo-PGF/sub 1..cap alpha../ were elevated toward the cervical end of the myometrium in ACTH-treated animals. PGF and PGE concentrations were higher at the tubal and cervical ends of the myometrium in both groups of animals. In the endometrium of treated animals, concentrations of PGE, but not PGF or 6-oxo-PGF/sub 1..cap alpha../, were elevated significantly. For cotyledons and chorioallantois, concentrations of PGF and 6-oxo-PGF/sub 1..cap alpha../, but not PGE, were elevated significantly in ACTH-treated animals. PG concentrations were not elevated in amnion taken from ACTH-treated animals. For both groups, the areas of highest PG concentrations were cotyledons and chorioallantois, suggesting that these may be major sites of synthesis.

• Research Organization: Univ. of Western Ontario, London, Canada
• OSTI ID: 5682019
• Journal Information: Endocrinology; (United States), Vol. 109:5
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ACTH
BIOLOGICAL EFFECTS
PROSTAGLANDINS
RADIOIMMUNOASSAY
ESTRADIOL
ESTRONE
EXPERIMENTAL DATA
FETUSES
HYDROCORTISONE
PARTURITION
PREGNANCY
PROGESTERONE
SHEEP
UTERUS
ADRENAL HORMONES
ANIMALS
BODY
CORTICOSTEROIDS
DATA
DOMESTIC ANIMALS
ESTRANES
ESTROGENS
FEMALE GENITALS
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
INFORMATION
ISOTOPE APPLICATIONS
KETONES
MAMMALS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PREGNANES
RADIOASSAY
RUMINANTS
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics