Autoradiographic localization of (/sup 125/I)-angiotensin II binding sites in the rat adrenal gland

Journal Article · Fri Mar 01 00:00:00 EST 1985 · Endocrinology; (United States)

OSTI ID:5681508

Healy, D P; Maciejewski, A R; Printz, M P

To gain greater insight into sites of action of circulating angiotensin II (Ang II) within the adrenal, we have localized the (/sup 125/I)-Ang II binding site using in vitro autoradiography. Autoradiograms were generated either by apposition of isotope-sensitive film or with emulsion-coated coverslips to slide-mounted adrenal sections labeled in vitro with 1.0 nM (/sup 125/I)-Ang II. Analysis of the autoradiograms showed that Ang II binding sites were concentrated in a thin band in the outer cortex (over the cells of the zona glomerulosa) and in the adrenal medulla, which at higher power was seen as dense patches. Few sites were evident in the inner cortex. The existence of Ang II binding sites in the adrenal medulla was confirmed by conventional homogenate binding techniques which revealed a single class of high affinity Ang II binding site (K/sub d/ . 0.7nM, B/sub max/ . 168.7 fmol/mg). These results suggest that the adrenal medulla may be a target for direct receptor-mediated actions of Ang II.

• Research Organization: Univ. of California, San Diego, La Jolla
• OSTI ID: 5681508
• Journal Information: Endocrinology; (United States), Vol. 116:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANGIOTENSIN
BIOCHEMICAL REACTION KINETICS
RECEPTORS
TISSUE DISTRIBUTION
ADRENAL GLANDS
AUTORADIOGRAPHY
IODINE 125
MALES
RATS
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CARDIOVASCULAR AGENTS
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
GLANDS
GLOBULINS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
VASOCONSTRICTORS
VERTEBRATES
550301* - Cytology- Tracer Techniques
550501 - Metabolism- Tracer Techniques