Photocontrol of gibberellin metabolism in situ in maize. [Zea mays L]

Journal Article · Sat Feb 01 00:00:00 EST 1986 · Plant Physiol.; (United States)

OSTI ID:5681243

Rood, S B; Beall, F D; Pharis, R P

Mature maize seeds were labeled with 10 to 100 pg per seed of (/sup 3/H) gibberellins (GA) and (/sup 3/H)GA glucosyl conjugate-like substances by feeding (/sup 3/H)GA/sub 20/ of high specific activity (2.3 Curies per millimole) during seed maturation. The dry seeds, which contained 14% (/sup 3/H)GA/sub 20/, 7% putative (/sup 3/H)GA/sub 1/ and 78% (/sup 3/H)GA glucosyl conjugate-like metabolites, were imbibed and germinated in the dark and under incandescent light. In both light and dark the proportion of (/sup 3/H)GA conjugate-like metabolities declined (relative to that in the mature dry seeds) during imbibition and up to germination at hour 36. This decline was accompanied by increases in the proportions of (/sup 3/H)GA/sub 20/ and putative (/sup 3/H)GA/sub 1/ thereby indicating hydrolysis, which was greater in the dark than in the light. The proportions of (/sup 3/H)GA conjugate-like substances in light-grown germinants were higher (121 and 141% of dark-grown) at 24 and 48 hour harvests and this statistically significant pattern was sustained up to 120 hours after imbibition. Conversely, the proportions of (/sup 3/H)GA/sub 20/ and putative (/sup 3/H)GA/sub 1/ were lower in the light-grown seedlings. Thus, during imbibition, hydrolysis (de-conjugation) of (/sup 3/H)GA glucosyl conjugate-like substances apparently occurred, and occurred more rapidly in the dark than in the light. Subsequently, during germination the reformation of (/sup 3/H)GA conjugate-like substances was less rapid in the dark than in the light.

• Research Organization: Univ. of Lethbridge, Alberta
• OSTI ID: 5681243
• Journal Information: Plant Physiol.; (United States), Vol. 80:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GIBBERELLIC ACID
METABOLISM
MAIZE
GERMINATION
VISIBLE RADIATION
BIOLOGICAL EFFECTS
COMPLEXES
EXPERIMENTAL DATA
HOMEOSTASIS
HYDROLYSIS
METABOLITES
PHOTOPERIOD
RESPONSE MODIFYING FACTORS
SEEDS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
CARBOXYLIC ACIDS
CEREALS
CHEMICAL REACTIONS
DATA
DECOMPOSITION
ELECTROMAGNETIC RADIATION
ESTERS
GRASS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LACTONES
LYSIS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
RADIATIONS
SOLVOLYSIS
550501* - Metabolism- Tracer Techniques