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Title: Angiotensin effects on calcium and steroidogenesis in adrenal glomerulosa cells

Abstract

We investigated the role of cellular calcium pools in angiotensin II-stimulated aldosterone synthesis in bovine adrenal glomerulosa cells. Angiotensin II decreased the size of the exchangeable cell calcium pool by 34%, consistent with previous observations that angiotensin II causes decreased uptake of /sup 45/Ca+2 into cells and increased efflux of /sup 45/Ca+2 from preloaded cells. Atomic absorption spectroscopy showed that angiotension II caused a decrease of 21% in total cellular calcium. Angiotensin II caused efflux of /sup 45/Ca+2 in the presence of EGTA and retarded uptake of /sup 45/Ca+2 when choline was substituted for sodium, suggesting that hormone effects on calcium pools do not involve influx of trigger calcium or sodium. Cells incubated in calcium-free buffer and 0.1 mM or 0.5 mM EGTA synthesized reduced (but still significant) amounts of the steroid in response to hormone. Cells incubated in increasing concentrations of extracellular calcium contained increasing amounts of intracellular calcium and synthesized increasing amounts of aldosterone in response to angiotensin II. These results point to the participation of intracellular calcium pools in angiotensin II-stimulated steroidogenesis and the importance of extracellular calcium in maintaining these pools.

Authors:
; ; ;
Publication Date:
Research Org.:
William S. Middleton Memorial Veterans Administration Hospital, Madison, WI
OSTI Identifier:
5681130
Resource Type:
Journal Article
Journal Name:
Endocrinology; (United States)
Additional Journal Information:
Journal Volume: 116:3
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ALDOSTERONE; BIOSYNTHESIS; ANGIOTENSIN; BIOLOGICAL FUNCTIONS; CALCIUM; ABSORPTION SPECTROSCOPY; ADRENAL GLANDS; CALCIUM 45; CELL CULTURES; CHOLINE; COWS; TRACER TECHNIQUES; ADRENAL HORMONES; ALCOHOLS; ALDEHYDES; ALKALINE EARTH ISOTOPES; ALKALINE EARTH METALS; AMINES; AMMONIUM COMPOUNDS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CALCIUM ISOTOPES; CARDIOVASCULAR AGENTS; CATTLE; CORTICOSTEROIDS; DAYS LIVING RADIOISOTOPES; DOMESTIC ANIMALS; DRUGS; ELEMENTS; ENDOCRINE GLANDS; EVEN-ODD NUCLEI; FUNCTIONS; GLANDS; GLOBULINS; HORMONES; HYDROXY COMPOUNDS; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; KETONES; LIPOTROPIC FACTORS; MAMMALS; METALS; MINERALOCORTICOIDS; NUCLEI; ORGANIC COMPOUNDS; ORGANS; PREGNANES; PROTEINS; QUATERNARY COMPOUNDS; RADIOISOTOPES; RUMINANTS; SPECTROSCOPY; STEROID HORMONES; STEROIDS; SYNTHESIS; VASOCONSTRICTORS; VERTEBRATES; 550501* - Metabolism- Tracer Techniques

Citation Formats

Elliott, M E, Siegel, F L, Hadjokas, N E, and Goodfriend, T L. Angiotensin effects on calcium and steroidogenesis in adrenal glomerulosa cells. United States: N. p., 1985. Web. doi:10.1210/endo-116-3-1051.
Elliott, M E, Siegel, F L, Hadjokas, N E, & Goodfriend, T L. Angiotensin effects on calcium and steroidogenesis in adrenal glomerulosa cells. United States. https://doi.org/10.1210/endo-116-3-1051
Elliott, M E, Siegel, F L, Hadjokas, N E, and Goodfriend, T L. 1985. "Angiotensin effects on calcium and steroidogenesis in adrenal glomerulosa cells". United States. https://doi.org/10.1210/endo-116-3-1051.
@article{osti_5681130,
title = {Angiotensin effects on calcium and steroidogenesis in adrenal glomerulosa cells},
author = {Elliott, M E and Siegel, F L and Hadjokas, N E and Goodfriend, T L},
abstractNote = {We investigated the role of cellular calcium pools in angiotensin II-stimulated aldosterone synthesis in bovine adrenal glomerulosa cells. Angiotensin II decreased the size of the exchangeable cell calcium pool by 34%, consistent with previous observations that angiotensin II causes decreased uptake of /sup 45/Ca+2 into cells and increased efflux of /sup 45/Ca+2 from preloaded cells. Atomic absorption spectroscopy showed that angiotension II caused a decrease of 21% in total cellular calcium. Angiotensin II caused efflux of /sup 45/Ca+2 in the presence of EGTA and retarded uptake of /sup 45/Ca+2 when choline was substituted for sodium, suggesting that hormone effects on calcium pools do not involve influx of trigger calcium or sodium. Cells incubated in calcium-free buffer and 0.1 mM or 0.5 mM EGTA synthesized reduced (but still significant) amounts of the steroid in response to hormone. Cells incubated in increasing concentrations of extracellular calcium contained increasing amounts of intracellular calcium and synthesized increasing amounts of aldosterone in response to angiotensin II. These results point to the participation of intracellular calcium pools in angiotensin II-stimulated steroidogenesis and the importance of extracellular calcium in maintaining these pools.},
doi = {10.1210/endo-116-3-1051},
url = {https://www.osti.gov/biblio/5681130}, journal = {Endocrinology; (United States)},
number = ,
volume = 116:3,
place = {United States},
year = {Fri Mar 01 00:00:00 EST 1985},
month = {Fri Mar 01 00:00:00 EST 1985}
}