Reactive oxygen metabolites produced by the carcinogenic fibrous mineral erionite

Journal Article · Fri Feb 01 00:00:00 EST 1991 · Environmental Research; (United States)

DOI:https://doi.org/10.1016/S0013-9351(05)80195-1· OSTI ID:5679044

Urano, Naoko; Yano, Eiji ^[1]; Evans, P H ^[2]

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1. Teikyo Univ. (Japan)
2. MRC Dunn Nutrition Unit, Cambridge (United Kingdom)

Erionite, a fibrous mineral and the causative agent of the endemic outbreak of mesothelioma in Turkey, has been shown to generate reactive oxygen metabolites (ROM) from polymorphonuclear leukocytes (PMN). In order to investigate the mechanism of the production of ROM by erionite from PMN, a luminol-dependent chemiluminescence (CL) method was utilized. Human peripheral blood PMN were incubated with 50-800 {mu}g/ml of erionite. PMN CL was produced immediately after the addition of erionite; the maximal CL production was reached within 2 to 6 minutes and the CL response increased with the dose of erionite. Superoxide dismutase, catalase, and dimethyl sulfoxide were utilized as scavengers of O{sub 2}, H{sub 2}O{sub 2}, and OH, respectively. These scavengers inhibited the production of erionite-stimulated PMN CL dose dependently, thus indicating the production of O{sub 2}{sup {minus}}, H{sub 2}O{sub 2}, and OH by erionite-stimulated PMN. The less phagocytically active cells, namely, mononuclear cells and erythrocytes, produced CL immediately after the addition of erionite or phorbol myristate acetate and displayed a significant delay period after the addition of zymosan. Therefore, the direct interaction between the cell surface membrane and erionite would appear to be more important than phagocytosis, per se, for the production of ROM by erionite.

• OSTI ID: 5679044
• Journal Information: Environmental Research; (United States), Vol. 54:1; ISSN 0013-9351
• Country of Publication: United States
• Language: English

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