The time course of ozone-induced neutrophilia in the proximal and distal airways of normal humans
- Univ. of California, Davis (United States)
It has been demonstrated that the first aliquot used in sequential bronchoalveolar lavage (3 {times} 60 ml) washes more proximal conducting airways (PA), and that subsequent aliquots sample from more distal airways and alveolar surfaces. The authors studied the time course of ozone (O{sub 3}) induced neutrophilia in the PA and DAAS of 5 normal exercising (V{sub E} = 60 1 min{sup {minus}1}) human subjects exposed for 1 h to filtered air (FA) once and to 0.3 ppm ozone (O{sub 3}) on 3 separate days. Bronchoalveolar lavage fluid was obtained < 1 after FA and either < 1, 6, or 24 h after O{sub 3} exposure. FEV{sub 1.0} was measured before the exposures and lavages. Four 60 ml aliquots of 0.9% NaCl solution were sequentially instilled into and withdrawn from a subsegment of the right middle lobe. The first aliquot (PA sample) was analyzed separately from poled aliquots 2 thru 4 (DAAS sample). FEV{sub 1.0} was significantly decreased < 1 h following O{sub 3} exposure, but returned to pre-exposure values at 6 and 24 h after O{sub 3}. The percent of neutrophils in the PA sample were significantly elevated at 6 h (16.5%) and 24 h (9.2%) after O{sub 3}. The percent of neutrophils in the DAAS sample were significantly elevated at 6 h (4.1%) and 24 h (5.1%) after O{sub 3}. PA vs DAAS comparisons were not significant at < 1 h after FA or O{sub 3} (1.1% vs 1.1% and 3.7% vs. 1.8%, respectively), but were significant 6 h and 24 h after O{sub 3}. These data demonstrate O{sub 3} induced FEV{sub 1.0} decrements and airway neutrophilia follow different time courses and suggest that O{sub 3} induced neutrophilia is greater in the PA compared to the DAAS and that PA neutrophilia peaks and begins to recover sooner.
- OSTI ID:
- 5678830
- Report Number(s):
- CONF-9004153-; CODEN: FAJOE
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 4:3; Conference: 74. annual meeting of the Federation of American Societies for Experimental Biology, Washington, DC (United States), 1-5 Apr 1990; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
Similar Records
Bilateral vagotomy or atropine pre-treatment reduces experimental diesel-soot induced lung inflammation
Staphylococcal enterotoxin A regulates bone marrow granulocyte trafficking during pulmonary inflammatory disease in mice
Related Subjects
OZONE
HEALTH HAZARDS
BIOLOGICAL FUNCTIONS
LUNGS
MAN
NEUTROPHILS
TIME DEPENDENCE
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
HAZARDS
LEUKOCYTES
MAMMALS
MATERIALS
ORGANS
PRIMATES
RESPIRATORY SYSTEM
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology