Regulation of cellular manganese and manganese transport rates in the unicellular alga Chlamydomonas

Journal Article · Tue Jan 01 00:00:00 EST 1985 · Limnol. Oceanogr.; (United States)

DOI:https://doi.org/10.4319/lo.1985.30.1.0071· OSTI ID:5673396

Sunda, W G; Huntsman, S A

The cellular accumulation and uptake kinetics of manganese by Chlamydomonas sp. were studied in model chelate buffer systems. Cellular manganese concentrations and uptake rates were related to the computed free manganese ion concentration and were independent of the total or chelated manganese concentration. Cellular manganese was constant at about 1 mmol liter/sup -1/ of cellular volume at free manganese ion concentrations of 10/sup -7/ /sup 6/-10/sup -6/ /sup 3/ mol liter/sup -1/ and decreased below this range. Manganese uptake rates followed saturation kinetics and V/sub max/, but not K/sub s/, varied with the free manganese ion concentration in the growth medium. V/sub max/ appeared to be under negative feedback control and increased with decreasing manganese ion concentration. Variations of up to 30-fold in this parameter seemed to be instrumental in limiting the variation in cellular manganese to a sixfold range despite a 1000-fold variation in free manganese ion concentration in the growth medium.

• Research Organization: National Marine Fisheries Service, Beaufort, NC
• OSTI ID: 5673396
• Journal Information: Limnol. Oceanogr.; (United States), Vol. 30:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
MANGANESE COMPOUNDS
UPTAKE
MEMBRANE TRANSPORT
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
CHLAMYDOMONAS
SEAWATER
ALGAE
CHEMISTRY
HYDROGEN COMPOUNDS
KINETICS
MICROORGANISMS
OXYGEN COMPOUNDS
PLANTS
REACTION KINETICS
TRANSITION ELEMENT COMPOUNDS
UNICELLULAR ALGAE
WATER
560303* - Chemicals Metabolism & Toxicology- Plants- (-1987)