Analysis of protein gene products in cells with altered chromosome sets for the purpose of genetic mapping
- Medical Genetic Research Center, Moscow (Russian Federation)
Two-dimensional electrophoresis was used for analyzing proteins in hybrid cells that contained single human chromosomes (chromosome 5, chromosome 21, or chromosomes 5 and 21) against the background of the mouse genome. By comparing the protein patterns of hybrid and parent cells (about 1000 protein fractions for each kind of cell), five fractions among proteins of hybrid cells were supposedly identified as human proteins. The genes of two of them are probably located on chromosome 5, and those of the other three on chromosome 21. Moreover, analysis of proteins in fibroblasts of patients with the cri-du-chat syndrome (5p-) revealed a decrease in the content of two proteins as compared with those in preparations of diploid fibroblasts. This fact was regarded as evidence that two corresponding genes are located on the short arm of chromosome 5. Methodological problems associated with the use of protein pattern analysis in cells with altered chromosome sets for the purposes of genetic mapping are discussed.
- OSTI ID:
- 56716
- Journal Information:
- Russian Journal of Genetics, Vol. 30, Issue 12; Other Information: PBD: Dec 1994; TN: Translated from Genetika; 30: No. 12, 1573-1581(1994)
- Country of Publication:
- United States
- Language:
- English
Similar Records
Physical mapping in the Cri du Chat region on human chromosome 5
The identification of five novel genes in the cri-du-chat critical region