Radioresistance of culture-induced augmented natural killer-like activity
Human NK activity is radiosensitive under the control of X-linked genes. We have evaluated the expression of these genes in other forms of cellular cytotoxicity. The NK radioresistant and radiosensitive phenotype is expressed in ADCC. Specific cellular cytotoxicity, generated in a MLC with a radiosensitive donor as responder, was radioresistant. NK-like activity recruited from nonadherent cells of radiosensitive subjects stimulated with allogenic cells, mitogens (PHA, Con A or PWM), or recall antigens (TT or PPD) was radioresistant. The acquisition of radioresistance was relatively rapid, beginning within 24 hr after exposure to PHA, prior to detectable proliferation. Radioresistance of MLR augmented NK-like activity was maximal 3 days after initiation of the culture. MLR augmented NK-like activity was spared by the immunosuppressive polypeptide antibiotic CsA at doses up to 1 mu gm/ml. CsA did, however, reduce acquisition of radioresistance by the NK-like activity at doses above 0.01 mu gm/ml, a concentration which does not inhibit uptake of /sup 3/H-thymidine but does reduce the level of specific CML. These data suggest that mitogens and antigens, including allogeneic cells, are recruiting radioresistant NK-like activity which can be distinguished from the radiosensitive spontaneous NK activity of the cell donor. Further, in the MLR, both radiosensitive and radioresistant NK-like activity may be recruited.
- Research Organization:
- Department of Pediatrics, Case Western Reserve University, Cleveland, OH
- OSTI ID:
- 5671581
- Journal Information:
- Hum. Immunol.; (United States), Vol. 7:3
- Country of Publication:
- United States
- Language:
- English
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CELL CULTURES
RADIOSENSITIVITY
ANTIGENS
ANTIMITOTIC DRUGS
CONCANAVALIN
INTERFERON
MAN
PHYTOHEMAGGLUTININ
TRACER TECHNIQUES
TRITIUM COMPOUNDS
X CHROMOSOME
AGGLUTININS
ANIMALS
ANTIBODIES
CARBOHYDRATES
CHROMOSOMES
DRUGS
HEMAGGLUTININS
HETEROCHROMOSOMES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MITOGENS
MUCOPROTEINS
ORGANIC COMPOUNDS
POLYSACCHARIDES
PRIMATES
PROTEINS
SACCHARIDES
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)