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Title: In vivo production of macrophage migration inhibition and stimulation factors during the inductive phase of the alloimmune response

Abstract

This paper offers a study of the production of macrophage migration inhibition factor (MIF), and also of the alternative macrophage migration stimulation factor (MSF), in vivo. Mice were injected with mouse spleen cells, irradiated with a dose of 1500 rads. The animals were divided into three groups, two of which were injected for a second time with irradiated mouse spleen cells. Samples of all fractions obtained by electrophoresis of sera of unimmunized mice had no significant effect of macrophage migration, while unfractionated sera of immunized mice obtained after a second injection of alloantigen as a rule stimulated macrophage migration. The results are evidence that T cells may function in vivo during the period before development of the antigen-specific proliferative response of T cells. The technique used to approach the problem, described in this study, can be used for preparative isolation of purified MIF and MSF without contamination by embryonic calf serum proteins which are usually present in culture in vitro.

Authors:
; ;
Publication Date:
Research Org.:
All-Union Oncologic Research Center, Moscow, USSR
OSTI Identifier:
5665044
Resource Type:
Journal Article
Journal Name:
Bull. Exp. Biol. Med. (Engl. Transl.); (United States)
Additional Journal Information:
Journal Volume: 102:7; Other Information: Translated from Byulleten' Ehksperimental'noj Biologii i Meditsiny; 102: No. 7, 66-68(Jul 1986)
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; MACROPHAGES; IMMUNE REACTIONS; MIGRATION; SPLEEN CELLS; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; ANTIGENS; BIOSYNTHESIS; ELECTROPHORESIS; EXTERNAL IRRADIATION; IMMUNE SERUMS; IMMUNOGLOBULINS; IN VIVO; INHIBITION; INTRAVENOUS INJECTION; LYMPHOCYTES; MICE; RESPONSE MODIFYING FACTORS; STIMULATION; ANIMAL CELLS; ANIMALS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CONNECTIVE TISSUE CELLS; GLOBULINS; INJECTION; INTAKE; IRRADIATION; LEUKOCYTES; MAMMALS; MATERIALS; ORGANIC COMPOUNDS; PHAGOCYTES; PROTEINS; RADIATION EFFECTS; RODENTS; SOMATIC CELLS; SYNTHESIS; VERTEBRATES; 560152* - Radiation Effects on Animals- Animals; 550300 - Cytology

Citation Formats

Suslov, A P, Yazova, A K, and Berkova, N P. In vivo production of macrophage migration inhibition and stimulation factors during the inductive phase of the alloimmune response. United States: N. p., 1986. Web. doi:10.1007/BF00840002.
Suslov, A P, Yazova, A K, & Berkova, N P. In vivo production of macrophage migration inhibition and stimulation factors during the inductive phase of the alloimmune response. United States. https://doi.org/10.1007/BF00840002
Suslov, A P, Yazova, A K, and Berkova, N P. 1986. "In vivo production of macrophage migration inhibition and stimulation factors during the inductive phase of the alloimmune response". United States. https://doi.org/10.1007/BF00840002.
@article{osti_5665044,
title = {In vivo production of macrophage migration inhibition and stimulation factors during the inductive phase of the alloimmune response},
author = {Suslov, A P and Yazova, A K and Berkova, N P},
abstractNote = {This paper offers a study of the production of macrophage migration inhibition factor (MIF), and also of the alternative macrophage migration stimulation factor (MSF), in vivo. Mice were injected with mouse spleen cells, irradiated with a dose of 1500 rads. The animals were divided into three groups, two of which were injected for a second time with irradiated mouse spleen cells. Samples of all fractions obtained by electrophoresis of sera of unimmunized mice had no significant effect of macrophage migration, while unfractionated sera of immunized mice obtained after a second injection of alloantigen as a rule stimulated macrophage migration. The results are evidence that T cells may function in vivo during the period before development of the antigen-specific proliferative response of T cells. The technique used to approach the problem, described in this study, can be used for preparative isolation of purified MIF and MSF without contamination by embryonic calf serum proteins which are usually present in culture in vitro.},
doi = {10.1007/BF00840002},
url = {https://www.osti.gov/biblio/5665044}, journal = {Bull. Exp. Biol. Med. (Engl. Transl.); (United States)},
number = ,
volume = 102:7,
place = {United States},
year = {Mon Dec 01 00:00:00 EST 1986},
month = {Mon Dec 01 00:00:00 EST 1986}
}