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Title: Capsule production by Pseudomonas aeruginosa

Abstract

Mucoid strains of Pseudomonas aeruginosa, associated almost exclusively with chronic respiratory infections in patients with cystic fibrosis, possess a capsule composed of alginic acid similar to one produced by Azotobacter vinelandii. Recent reports have provided evidence that the biosynthetic pathway for alginate in P. aeruginosa may differ from the pathway proposed for A. vinelandii in that synthesis in P. aeruginosa may occur by way of the Entner-Doudoroff pathway. Incorporation of isotope from (6-/sup 14/C)glucose into alginate by both P. aueroginosa and A. vinelandii was 10-fold greater than that from either (1-/sup 14/C)/sup -/ or (2-/sup 14/C)glucose, indicating preferential utilization of the bottom half of the glucose molecule for alginate biosynthesis. These data strongly suggest that the Entner-Doudoroff pathway plays a major role in alginate synthesis in both P. aeruginosa and A. vinelandii. The enzymes of carbohydrate metabolism in mucoid strains of P. aeruginosa appear to be unchanged whether alignate is actively produced or not and activities do not differ significantly from nonmucoid strain PAO.

Authors:
Publication Date:
Research Org.:
Oklahoma Univ., Oklahoma City (USA). Health Sciences Center
OSTI Identifier:
5664163
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: Thesis (Ph. D.)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ALGINATES; BIOSYNTHESIS; AZOTOBACTER; ENCAPSULATION; PSEUDOMONAS; ALGINIC ACID; BIOLOGICAL PATHWAYS; CAPSULES; CARBON 14 COMPOUNDS; ENZYMES; GLUCOSE; TRACER TECHNIQUES; ALDEHYDES; BACTERIA; CARBOHYDRATES; COLLOIDS; CONTAINERS; DISPERSIONS; HEXOSES; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MICROORGANISMS; MONOSACCHARIDES; ORGANIC COMPOUNDS; POLYSACCHARIDES; SACCHARIDES; SYNTHESIS; 550701* - Microbiology- Tracer Techniques

Citation Formats

Lynn, A R. Capsule production by Pseudomonas aeruginosa. United States: N. p., 1984. Web.
Lynn, A R. Capsule production by Pseudomonas aeruginosa. United States.
Lynn, A R. 1984. "Capsule production by Pseudomonas aeruginosa". United States.
@article{osti_5664163,
title = {Capsule production by Pseudomonas aeruginosa},
author = {Lynn, A R},
abstractNote = {Mucoid strains of Pseudomonas aeruginosa, associated almost exclusively with chronic respiratory infections in patients with cystic fibrosis, possess a capsule composed of alginic acid similar to one produced by Azotobacter vinelandii. Recent reports have provided evidence that the biosynthetic pathway for alginate in P. aeruginosa may differ from the pathway proposed for A. vinelandii in that synthesis in P. aeruginosa may occur by way of the Entner-Doudoroff pathway. Incorporation of isotope from (6-/sup 14/C)glucose into alginate by both P. aueroginosa and A. vinelandii was 10-fold greater than that from either (1-/sup 14/C)/sup -/ or (2-/sup 14/C)glucose, indicating preferential utilization of the bottom half of the glucose molecule for alginate biosynthesis. These data strongly suggest that the Entner-Doudoroff pathway plays a major role in alginate synthesis in both P. aeruginosa and A. vinelandii. The enzymes of carbohydrate metabolism in mucoid strains of P. aeruginosa appear to be unchanged whether alignate is actively produced or not and activities do not differ significantly from nonmucoid strain PAO.},
doi = {},
url = {https://www.osti.gov/biblio/5664163}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 1984},
month = {Sun Jan 01 00:00:00 EST 1984}
}

Thesis/Dissertation:
Other availability
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