Early somatic embryo induction events in alfalfa callus cultures. [Medicago sativa]

Conference · Wed Apr 01 00:00:00 EST 1987 · Plant Physiol., Suppl.; (United States)

OSTI ID:5650948

El-Bakry, A A; Hildebrand, D F

High and low regenerating alfalfa Medicago sativa L. cv Regen S full sibs were isolated from a callus culture screen on modified Blaydes medium. The average number of embryos per ovary were thirty and zero for the high and low genotypes respectively after six weeks in culture. Proembryonic cell masses (4-8 celled) were observed after 4-5 days in culture and maximum meristematic activity was at 6-7 days in culture, for the high regenerating genotypes. Well formed globular embryos, both epidermal and subepidermal in origin, were observed after 2 weeks is culture. Samples in culture for 3, 6 and 14 days from the high and low regenerating genotypes were radiolabeled in vivo with /sup 35/S-methionine and run both on one and two dimension gels. The results will be discussed in relation to differences in proteins between the high and low regenerating genotypes at the stage of maximum meristematic activity (day 6) and differences occurring relative to the appearance of globular stage embryos (day 14) will be presented.

• Research Organization: Univ. of Kentucky, Lexington
• OSTI ID: 5650948
• Report Number(s): CONF-8707108-
• Journal Information: Plant Physiol., Suppl.; (United States), Vol. 83:4; Conference: Annual meeting of the American Society of Plant Physiologists, St. Louis, MO, USA, 19 Jul 1987
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ALFALFA
BIOLOGICAL REGENERATION
GENOTYPE
PROTEIN STRUCTURE
ELECTROPHORESIS
LABELLED COMPOUNDS
MERISTEMS
PLANT TISSUES
SULFUR 35
TRACER TECHNIQUES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL RECOVERY
BODY
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LEGUMINOSAE
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
PLANTS
RADIOISOTOPES
RECOVERY
RHIZOBIUM
SULFUR ISOTOPES
TISSUES
551001* - Physiological Systems- Tracer Techniques