Physiology of natural killer cells. In vivo regulation of progenitors by interleukin 3
Adoptive transfer of bone marrow cells to syngeneic lethally irradiated C57BL/6 mice was used to study the maturation of natural killer (NK) cells from their progenitors. The NK progenitor cell was found to be asialomonoganglioside-negative, (aGM1-) Thy-1-, NK-1-, Ly-1-, Ly-2-, and L3T4-. The NK cells emerging from the bone marrow grafts were aGM1+, NK-1+, Thy-1+/-, Ly-1-, Ly-2-, and L3T4- and to have a target specter similar to that of NK cells isolated from the spleen of normal mice. The regulatory role of interleukin 2 (IL-2) and interleukin 3 (IL-3) for the maturation of NK cells was examined by exposure of the bone marrow cells to the lymphokines in vitro before bone marrow grafting or by treatment of bone marrow-grafted mice with lymphokines through s.c. implanted miniosmotic pumps. IL-3 antagonized the IL-2-induced maturation of NK cells in vitro and strongly inhibited the generation of NK cells after adoptive transfer of bone marrow cells in vivo. The suppressive effect of IL-3 was evident throughout the treatment period (8 or 16 days) but was apparently reversible because NK activity returned to control levels within 8 days after cessation of treatment. The inhibition of cytotoxic activity was accompanied by a reduced appearance of cells with the NK phenotypic markers aGM1 or NK-1, indicating that not only the cytotoxic activity of NK cells but also their actual formation was inhibited. Concomitantly, a moderate increase in cells expressing the T cell marker L3T4 and an increased proliferative response to the T cell mitogen concanavalin A was observed. A direct estimate of the effect of IL-3 on the frequency of NK cell progenitors was obtained by limiting dilution analysis of bone marrow cells at day 8 after bone marrow transplantation.
- Research Organization:
- Univ. of Lund, Sweden
- OSTI ID:
- 5650209
- Journal Information:
- J. Immunol.; (United States), Vol. 139:11
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BONE MARROW CELLS
TRANSPLANTS
LYMPHOCYTES
CELL DIFFERENTIATION
PHYSIOLOGY
LYMPHOKINES
BIOLOGICAL FUNCTIONS
CELL CULTURES
CELL KILLING
CONCANAVALIN
IN VIVO
LETHAL IRRADIATION
MICE
RADIATION CHIMERAS
STEM CELLS
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHIMERAS
CONNECTIVE TISSUE CELLS
FUNCTIONS
GROWTH FACTORS
HEMAGGLUTININS
IRRADIATION
LECTINS
LEUKOCYTES
MAMMALS
MATERIALS
MITOGENS
MOSAICISM
ORGANIC COMPOUNDS
PROTEINS
RODENTS
SOMATIC CELLS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals