Stimulation of neutrophils by tumor necrosis factor
Human recombinant tumor necrosis factor (TNF) was shown to be a weak direct stimulus of the neutrophil respiratory burst and degranulation. The stimulation, as measured by iodination, H/sub 2/O/sub 2/ production, and lysozyme release, was considerably increased by the presence of unopsonized zymosan in the reaction mixture, an effect which was associated with the increased ingestion of the zymosan. TNF does not act as an opsonin but, rather, reacts with the neutrophil to increase its phagocytic activity. TNF-dependent phagocytosis, as measured indirectly by iodination, is inhibited by monoclonal antibodies (Mab) 60.1 and 60.3, which recognize different epitopes on the C3bi receptor/adherence-promoting surface glycoprotein of neutrophils. Other neutrophil stimulants, namely N-formyl-methionyl-leucyl-phenylalanine, the Ca2+ ionophore A23187, and phorbol myristic acetate, also increase iodination in the presence of zymosan; as with TNF, the effect of these stimulants is inhibited by Mab 60.1 and 60.3, whereas, in contrast to that of TNF, their stimulation of iodination is unaffected by an Mab directed against TNF. TNF may be a natural stimulant of neutrophils which promotes adherence to endothelial cells and to particles, leading to increased phagocytosis, respiratory burst activity, and degranulation.
- Research Organization:
- Univ. of Washington, Seattle
- OSTI ID:
- 5649587
- Journal Information:
- J. Immunol.; (United States), Vol. 11
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
NEUTROPHILS
PHAGOCYTOSIS
PHENYLALANINE
BIOLOGICAL EFFECTS
PHORBOL ESTERS
ZYMOSAN
GLUCOPROTEINS
HYDROGEN PEROXIDE
IODINE ISOTOPES
LACTATE DEHYDROGENASE
LYSOZYME
MONOCLONAL ANTIBODIES
NECROSIS
NEOPLASMS
TRACER TECHNIQUES
AMINO ACIDS
ANTIBODIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARBOXYLIC ACIDS
CARCINOGENS
DISEASES
ENZYMES
ESTERS
GLYCOSYL HYDROLASES
HEMIACETAL DEHYDROGENASES
HYDROGEN COMPOUNDS
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MATERIALS
O-GLYCOSYL HYDROLASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PATHOLOGICAL CHANGES
PEROXIDES
PROTEINS
SACCHARIDES
560306* - Chemicals Metabolism & Toxicology- Man- (-1987)
550901 - Pathology- Tracer Techniques