Intestinal absorption and tissue distribution of ( sup 14 C)pyrroloquinoline quinone in mice

Journal Article · Wed May 01 00:00:00 EDT 1991 · Proceedings of the Society for Experimental Biology and Medicine; (USA)

OSTI ID:5642978

Smidt, C R; Unkefer, C J; Houck, D R; Rucker, R B ^[1]

---

1. Univ. of California, Davis (USA)

Pyrroloquinoline quinone (PQQ) functions as a cofactor for prokaryotic oxidoreductases, such as methanol dehydrogenase and membrane-bound glucose dehydrogenase. In animals fed chemically defined diets, PQQ improves reproductive outcome and neonatal growth. Consequently, the present study was undertaken to determine the extent to which PQQ is absorbed by the intestine, its tissue distribution, and route of excretion. About 28 micrograms of PQQ (0.42 microCi/mumol), labeled with {sup 14}C derived from L-tyrosine, was administered orally to Swiss-Webster mice (18-20 g) to estimate absorption. PQQ was readily absorbed (62%, range 19-89%) in the lower intestine, and was excreted by the kidneys (81% of the absorbed dose) within 24 hr. The only tissues that retained significant amounts of ({sup 14}C)PQQ at 24 hr were skin and kidney. For kidney, it was assumed that retention of ({sup 14}C)PQQ represented primarily PQQ destined for excretion. For skin, the concentration of ({sup 14}C)PQQ increased from 0.3% of the absorbed dose at 6 hr to 1.3% at 24 hr. Furthermore, most of the ({sup 14}C)PQQ in blood (greater than 95%) was associated with the blood cell fraction, rather than plasma.

• OSTI ID: 5642978
• Journal Information: Proceedings of the Society for Experimental Biology and Medicine; (USA), Vol. 197:1; ISSN 0037-9727
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
QUINONES
METABOLISM
CARBON 14 COMPOUNDS
COENZYMES
ERYTHROCYTES
INTESTINAL ABSORPTION
ISOTOPE DILUTION
LIVER
MICE
PSEUDOMONAS
QUINOLINES
SKIN
TISSUE DISTRIBUTION
ABSORPTION
ANIMALS
AROMATICS
AZAARENES
AZINES
BACTERIA
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBON COMPOUNDS
DIGESTIVE SYSTEM
DISTRIBUTION
GLANDS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
PYRIDINES
RODENTS
TRACER TECHNIQUES
UPTAKE
VERTEBRATES
550501* - Metabolism- Tracer Techniques