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Title: Nicotine inhibits collagen synthesis and alkaline phosphatase activity, but stimulates DNA synthesis in osteoblast-like cells

Use of smokeless tobacco is associated with various oral lesions including periodontal damage and alveolar bone loss. This study was performed to test the effects of nicotine on bone-forming cells at concentrations that occur in the saliva of smokeless tobacco users. Confluent cultures of osteoblast-like cells isolated from chick embryo calvariae were incubated for 2 days with nicotine added to the culture medium (25-600 micrograms/ml). Nicotine inhibited alkaline phosphatase in the cell layer and released to the medium, whereas glycolysis (as indexed by lactate production) was unaffected or slightly elevated. The effects on medium and cell layer alkaline phosphatase were concentration dependent with maximal inhibition occurring at 600 micrograms nicotine/ml. Nicotine essentially did not affect the noncollagenous protein content of the cell layer, but did inhibit collagen synthesis (hydroxylation of ({sup 3}H)proline and collagenase-digestible protein) at 100, 300, and 600 micrograms/ml. Release of ({sup 3}H)hydroxyproline to the medium was also decreased in a dose-dependent manner, as was the collagenase-digestible protein for both the medium and cell layer. In contrast, DNA synthesis (incorporation of ({sup 3}H)thymidine) was more than doubled by the alkaloid, whereas total DNA content was slightly inhibited at 600 micrograms/ml, suggesting stimulated cell turnover. Morphologic changes occurred inmore » nicotine-treated cells including rounding up, detachment, and the occurrence of numerous large vacuoles. These results suggest that steps to reduce the salivary concentration of nicotine in smokeless tobacco users might diminish damaging effects of this product on alveolar bone.« less
Authors:
; ;  [1]
  1. (Univ. of Louisville, KY (USA))
Publication Date:
OSTI Identifier:
5642964
Resource Type:
Journal Article
Resource Relation:
Journal Name: Proceedings of the Society for Experimental Biology and Medicine; (USA); Journal Volume: 197:1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ALKALINE PHOSPHATASE; ENZYME ACTIVITY; BONE CELLS; DNA REPLICATION; COLLAGEN; BIOSYNTHESIS; NICOTINE; BIOLOGICAL EFFECTS; CHICKENS; DOSE-RESPONSE RELATIONSHIPS; EMBRYOS; INHIBITION; PROLINE; THYMIDINE; TOBACCO PRODUCTS; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ALKALOIDS; AMINES; AMINO ACIDS; ANIMAL CELLS; ANIMALS; AUTONOMIC NERVOUS SYSTEM AGENTS; AZINES; AZOLES; BIRDS; CARBOXYLIC ACIDS; CONNECTIVE TISSUE CELLS; DRUGS; ENZYMES; ESTERASES; FOWL; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; HYDROLASES; ISOTOPE APPLICATIONS; NUCLEIC ACID REPLICATION; NUCLEOSIDES; NUCLEOTIDES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PARASYMPATHOLYTICS; PARASYMPATHOMIMETICS; PHOSPHATASES; PROTEINS; PYRIDINES; PYRIMIDINES; PYRROLES; PYRROLIDINES; RIBOSIDES; SCLEROPROTEINS; SOMATIC CELLS; SYNTHESIS; VERTEBRATES 550201* -- Biochemistry-- Tracer Techniques