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Title: Differential expression of murine adult hemoglobins in early ontogeny

A hemoglobin mutation is described that permits study of the expression of the two adult ..beta..-globin genes throughout fetal and postnatal development. Mice with a mutation at the Hbb/sup s/, ..beta..-globin locus, were used to study the relative levels of ..beta..-s2major and ..beta..-sminor globins specified by the mutant Hbb/sup s2/ haplotype during development. At 11.5 days of gestation ..beta..-sminor comprised over 80% and ..beta..-s2major under 20% of the adult beta-globin. The relative level of ..beta..-sminor decreased through fetal development; at birth ..beta..-sminor represented 33.7% of the ..beta..-globin. The adult values of 71.0% ..beta..-s2major and 29.0% ..beta..-sminor globin are expressed in mice six days after birth. Because the two ..beta..-globin genes are expressed in mice of the Hbb/sup 2s/ haplotype, both the ..beta..-smajor and ..beta..-sminor genes must be expressed in mice of the Hbb/sup s/ haplotype. Expression of the ..beta..-sminor gene is elevated to 35.6% in Hbb/sup s2/ mice that have been bled repeatedly. Thus, the 5' ..beta..-s2major and 3' ..beta..-sminor genes of the Hbb/sup s2/ haplotype and, presumably the 5' ..beta..-smajor and 3' ..beta..-sminor genes of the Hbb/sup s/ haplotype, are regulated independently and are homologous to the 5' ..beta..-dmajor and 3' ..beta..-dminor genes of the Hbb/sup d/ haplotype. Micemore » of the Hbb/sup s2/ haplotype are better than mice of the Hbb/sup d/ haplotytpe for studying the mechanisms of hemoglobin switching because the Hbb/sup s2/ each of the three embryonic and two adult hemoglobins can be separated by electrophoresis. 17 refs., 3 figs.« less
Authors:
; ;
Publication Date:
OSTI Identifier:
5642932
Report Number(s):
CONF-8409198-1
ON: DE85005364
DOE Contract Number:
AC05-84OR21400
Resource Type:
Conference
Resource Relation:
Conference: Conference on hemoglobin switching, Airlie, VA, USA, 30 Sep 1984
Research Org:
Tennessee Univ., Oak Ridge (USA). Graduate School of Biomedical Sciences; Research Triangle Inst., Research Triangle Park, NC (USA). Chemistry and Life Sciences Div.; Oak Ridge National Lab., TN (USA)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BLOOD FORMATION; ONTOGENESIS; HEMOGLOBIN; GENES; QUANTITY RATIO; FETUSES; MICE; SICKLE CELL ANEMIA; THALASSEMIA; ANEMIAS; ANIMALS; CARBOXYLIC ACIDS; DISEASES; GLOBIN; HEMIC DISEASES; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; MAMMALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PIGMENTS; PORPHYRINS; PROTEINS; RODENTS; SYMPTOMS; VERTEBRATES 550800* -- Morphology