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Title: Complexity of the primary genetic response to mitogenic activation of human T cells

The authors describe the isolation and characterization of more than 60 novel cDNA clones that constitute part of the immediate genetic response to resting human peripheral blood T cells after mitogen activation. This primary response was highly complex, both in the absolute number of inducible genes and in the diversity of regulation. Although most of the genes expressed in activated T cells were shared with the activation response of normal human fibroblasts, a significant number were more restricted in tissue specificity and thus likely encode or effect the differentiated functions of activated T cells. The activatable genes could be further differentiated on the basis of kinetics of induction, response to cycloheximide, and sensitivity to the immunosuppressive drug cylcosporin A. It is of note that cyclosporin A inhibited the expression of more than 10 inducible genes, which suggests that this drug has a broad genetic mechanism of action.
Authors:
;  [1] ; ;  [2]
  1. (National Inst. of Allergy and Infectious Diseases, Bethesda, MD (USA))
  2. (National Cancer Inst., Bethesda, MD (USA))
Publication Date:
OSTI Identifier:
5641168
Resource Type:
Journal Article
Resource Relation:
Journal Name: Molecular and Cellular Biology; (USA); Journal Volume: 9:3
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CELL DIFFERENTIATION; GENE REGULATION; LYMPHOCYTES; GENE AMPLIFICATION; MITOGENS; BIOLOGICAL EFFECTS; CLASSIFICATION; CYCLOHEXIMIDE; DNA-CLONING; FIBROBLASTS; IMMUNOSUPPRESSION; IMMUNOSUPPRESSIVE DRUGS; SENSITIVITY; ANIMAL CELLS; ANTI-INFECTIVE AGENTS; ANTIBIOTICS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CLONING; CONNECTIVE TISSUE CELLS; DNA HYBRIDIZATION; DRUGS; FUNGICIDES; HYBRIDIZATION; LEUKOCYTES; MATERIALS; PESTICIDES; SOMATIC CELLS 550400* -- Genetics; 550300 -- Cytology; 560300 -- Chemicals Metabolism & Toxicology