Electrical and freeze-fracture analysis of the effects of ionic cadmium on cell membranes of human proximal tubule cells
Abstract
The authors previously reported that cell cultures of human proximal tubule (HPT) cells respond to ionic cadmium in a manner consistent with well-defined Cd[sup 2+]-elicited responses reported for in vivo systems. However, one unique finding was that the transepithelial electrical resistance and tight junction sealing strands were altered as a result of Cd[sup 2+] exposure at micromolar concentrations. These alterations are reexamined in detail in the present report to determine whether the Cd[sup 2+]-induced alterations are specific alterations in the tight junction structure or reflect a general alteration in the cell membrane. Exhaustive analysis of tight junction sealing strands demonstrated no significant alterations due to Cd[sup 2+] exposure, even at the concentration that elicited a significant reduction in transepithelial resistance. Further analysis of intramembrane particle distribution demonstrated a significant increase in apical intramembrane particles, indicating that Cd[sup 2+] exposure altered the characteristics of the apical cell membrane. Overall, the results were consistent with evidence of Cd[sup 2+]-induced alteration in the apical cell membrane of the HPT cell.
- Authors:
-
- Medical Univ. of South Carolina, Charleston, SC (United States)
- Publication Date:
- OSTI Identifier:
- 5640583
- Resource Type:
- Journal Article
- Journal Name:
- Environmental Health Perspectives; (United States)
- Additional Journal Information:
- Journal Volume: 101:6; Journal ID: ISSN 0091-6765
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; CADMIUM; TOXICITY; CELL MEMBRANES; ELECTRIC CONDUCTIVITY; TUBULES; CELL CULTURES; BIOELECTRICITY; BIOLOGICAL EFFECTS; ELECTROPHYSIOLOGY; JUNCTIONS; KIDNEYS; BODY; CELL CONSTITUENTS; ELECTRICAL PROPERTIES; ELECTRICITY; ELEMENTS; MEMBRANES; METALS; ORGANS; PHYSICAL PROPERTIES; PHYSIOLOGY; 560300* - Chemicals Metabolism & Toxicology; 550200 - Biochemistry
Citation Formats
Hazen-Martin, D J, Todd, J H, Sens, M A, Khan, W, Bylander, J E, Smyth, B J, and Sens, D A. Electrical and freeze-fracture analysis of the effects of ionic cadmium on cell membranes of human proximal tubule cells. United States: N. p., 1993.
Web. doi:10.1289/ehp.93101510.
Hazen-Martin, D J, Todd, J H, Sens, M A, Khan, W, Bylander, J E, Smyth, B J, & Sens, D A. Electrical and freeze-fracture analysis of the effects of ionic cadmium on cell membranes of human proximal tubule cells. United States. https://doi.org/10.1289/ehp.93101510
Hazen-Martin, D J, Todd, J H, Sens, M A, Khan, W, Bylander, J E, Smyth, B J, and Sens, D A. 1993.
"Electrical and freeze-fracture analysis of the effects of ionic cadmium on cell membranes of human proximal tubule cells". United States. https://doi.org/10.1289/ehp.93101510.
@article{osti_5640583,
title = {Electrical and freeze-fracture analysis of the effects of ionic cadmium on cell membranes of human proximal tubule cells},
author = {Hazen-Martin, D J and Todd, J H and Sens, M A and Khan, W and Bylander, J E and Smyth, B J and Sens, D A},
abstractNote = {The authors previously reported that cell cultures of human proximal tubule (HPT) cells respond to ionic cadmium in a manner consistent with well-defined Cd[sup 2+]-elicited responses reported for in vivo systems. However, one unique finding was that the transepithelial electrical resistance and tight junction sealing strands were altered as a result of Cd[sup 2+] exposure at micromolar concentrations. These alterations are reexamined in detail in the present report to determine whether the Cd[sup 2+]-induced alterations are specific alterations in the tight junction structure or reflect a general alteration in the cell membrane. Exhaustive analysis of tight junction sealing strands demonstrated no significant alterations due to Cd[sup 2+] exposure, even at the concentration that elicited a significant reduction in transepithelial resistance. Further analysis of intramembrane particle distribution demonstrated a significant increase in apical intramembrane particles, indicating that Cd[sup 2+] exposure altered the characteristics of the apical cell membrane. Overall, the results were consistent with evidence of Cd[sup 2+]-induced alteration in the apical cell membrane of the HPT cell.},
doi = {10.1289/ehp.93101510},
url = {https://www.osti.gov/biblio/5640583},
journal = {Environmental Health Perspectives; (United States)},
issn = {0091-6765},
number = ,
volume = 101:6,
place = {United States},
year = {Mon Nov 01 00:00:00 EST 1993},
month = {Mon Nov 01 00:00:00 EST 1993}
}