Evaluation of genotoxic effects of the herbicide dicamba using in vivo and in vitro test systems

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Environmental and Molecular Mutagenesis; (USA)

DOI:https://doi.org/10.1002/em.2850150303· OSTI ID:5639030

Perocco, P; Ancora, G; Rani, P; Valenti, A M; Mazzullo, M; Colacci, A; Grilli, S ^[1]

---

1. Universita di Bologna (Italy)

The genotoxic effects of the herbicide dicamba have been studied by measuring (1) the unwinding rate of liver DNA from intraperitoneally treated rats (fluorimetric assay); (2) DNA repair as unscheduled DNA synthesis (UDS) induced in cultured human peripheral blood lymphocytes (HPBL); and (3) sister chromatid exchanges (SCE) in HPBL. Results show that dicamba is capable of inducing DNA damage since it significantly increases the unwinding rate of rat liver DNA in vivo and also induces UDS in HPBL in vitro in the presence of exogenous metabolic activation (S-9 mix). Furthermore, dicamba causes a very slight increase in SCE frequency in HPBL in vitro.

• OSTI ID: 5639030
• Journal Information: Environmental and Molecular Mutagenesis; (USA), Vol. 15:3; ISSN 0893-6692
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
BENZOIC ACID
BIOLOGICAL EFFECTS
HERBICIDES
DNA REPLICATION
DNA REPAIR
IN VIVO
LIVER
LYMPHOCYTES
METABOLIC ACTIVATION
RATS
SISTER CHROMATID EXCHANGES
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
CHROMOSOMAL ABERRATIONS
CONNECTIVE TISSUE CELLS
DIGESTIVE SYSTEM
GLANDS
HYDROGEN COMPOUNDS
LEUKOCYTES
MAMMALS
MATERIALS
MONOCARBOXYLIC ACIDS
MUTATIONS
NUCLEIC ACID REPLICATION
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PESTICIDES
RECOVERY
REPAIR
RODENTS
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques