Quantitative analysis of disturbed cell maturation in dysplastic lesions of the respiratory tract epithelium
Autoradiographic patterns of (/sup 3/H)thymidine incorporation, nuclear/cytoplasmic ratios (N/C), and the percentage of dark epithelial cells were analyzed in a group of epithelial lesions induced by 7,12-dimethylbenz(a)anthracene (DMBA) in rat tracheal transplants. It was found that similar lesions of different age exhibit the same labeling indices (LIs), therefore the lesions of different age were subsequently pooled in the following groups and studied by high resolution light microscopic autoradiography: squamous metaplasia without or with only mild atypia, squamous metaplasia with moderate atypia, squamous metaplasia with severe atypia, carcinoma in situ, and microinvasive carcinoma. Normal tracheal and esophageal epithelia were also analyzed. Gradients of increasing N/C (nucleus-cytoplasm ratios) values could be observed as the lesions increased in severity, especially in the middle and surface layers (e.g., in the surface layer regular metaplasia N/C = 0.08, squamous metaplasia with moderate atypia N/C = 0.26, and carcinoma in situ N/C = 0.50). Dark cells were absent in the normal esophageal epithelium, were present in moderate numbers in the basal layer of regular squamous metaplasia (18%), and increased markedly in the atypical epithelial lesions (approximately 50% in the atypical squamous metaplasias and 70% in carcinoma in situ). In the suprabasal layer dark cells increased from 3% in squamous metaplasia with moderate atypia to 28% in metaplasia with severe atypia and 56% in carcinoma in situ. The results confirm in a quantitative fashion that disturbances of cell maturation and cell proliferation are key features of dysplastic lesions induced by chemical carcinogens, and suggest the use of objective parameters for evaluation and classification of preneoplastic alterations.
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5637081
- Journal Information:
- Carcinogenesis (N.Y.); (United States), Vol. 1:12, Issue 12
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
DIMETHYLBENZANTHRACENE
BIOLOGICAL EFFECTS
GRAFTS
CARCINOGENESIS
AUTORADIOGRAPHY
CARCINOMAS
MORPHOLOGICAL CHANGES
RATS
THYMIDINE
TRACHEA
TRITIUM COMPOUNDS
ANIMALS
AROMATICS
AZINES
CONDENSED AROMATICS
DISEASES
HETEROCYCLIC COMPOUNDS
LABELLED COMPOUNDS
MAMMALS
NEOPLASMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOGENESIS
PYRIMIDINES
RESPIRATORY SYSTEM
RIBOSIDES
RODENTS
TRANSPLANTS
VERTEBRATES
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