Biosynthesized (/sup 35/S)methionine-labeled pro-opiomelanocortin peptides as novel recovery markers in radioimmunoassay of peptide hormones

Journal Article · Sun Dec 01 00:00:00 EST 1985 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5636830

Rosendale, B E; Jarrett, D B

Hormones are extracted from plasma with varying efficiency. Thus, markers or internal standards are often needed, to monitor and correct for extraction losses. To do so is difficult in the case of peptide hormones because radioactive recovery markers either have a low specific activity or, if labeled with iodine, may not be fully representative because of alterations in their size and charge. More importantly, markers labeled with /sup 125/I can interact in, and thus compromise, the subsequent radioimmunoassay. AtT-20 mouse pituitary tumor cells, which can be stimulated to synthesize and secrete pro-opiomelanocortin peptides, can biosynthetically label beta-lipotropin (beta-LPH) with (/sup 35/S)methionine. The labeled peptide, which is co-eluted with unlabeled beta-LPH in high-performance liquid chromatography, is fully immunoprecipitable and has a specific activity of 34 Ci/g. We use this labeled peptide to monitor the recovery of beta-LPH in silicic acid extraction from plasma. This peptide is an ideal marker of analytical recovery because it does not interfere in subsequent radioimmunoassays.

• OSTI ID: 5636830
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Vol. 12
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HORMONES
RADIOIMMUNOASSAY
PEPTIDES
LABELLING
BIOLOGICAL MARKERS
ENDORPHINS
IODINE 125
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
METHIONINE
MICE
SILICIC ACID
SULFUR 35
TRACER TECHNIQUES
AMINO ACIDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
HYDROGEN COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
INORGANIC ACIDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
NEUROREGULATORS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RODENTS
SEPARATION PROCESSES
SULFUR ISOTOPES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques