Optimization of fermentation conditions for alcohol production
The quantitative effects of carbohydrate levels, degree of initial saccharification, glucoamylase dosage, temperature, and fermentation time were investigated using a Box-Wilson central composite design protocol. With Saccharomyces cerevisiae ATCC 4126, it was found that the use of a partially saccharified starch substrate markedly increased yields and attainable alcohol levels. Balancing the degree of initial saccharification with the level of glucoamylase used to complete hydrolysis was found necessary to obtain optimum yields. The temperature optimum was found to be 36 degrees C. The regression equations obtained were used to model the fermentation in order to determine optimum fermentation conditions. 11 references.
- Research Organization:
- Amstar Corp., Dimmitt, TX
- OSTI ID:
- 5634255
- Journal Information:
- Biotechnol. Bioeng.; (United States), Vol. 26:12
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ETHANOL
BIOSYNTHESIS
STARCH
ENZYMATIC HYDROLYSIS
SACCHARIFICATION
FERMENTATION
OPTIMIZATION
REACTION KINETICS
REGRESSION ANALYSIS
SACCHAROMYCES CEREVISIAE
TEMPERATURE DEPENDENCE
ALCOHOLS
BIOCONVERSION
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
FUNGI
HYDROLYSIS
HYDROXY COMPOUNDS
KINETICS
LYSIS
MATHEMATICS
MICROORGANISMS
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
REAGENTS
SACCHARIDES
SACCHAROMYCES
SOLVOLYSIS
STATISTICS
SYNTHESIS
YEASTS
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