Formation of inositol 1,3,4,6-tetrakisphosphate during angiotensin II action in bovine adrenal glomerulosa cells
Angiotensin II stimulates the formation of several inositol polyphosphates in cultured bovine adrenal glomerulosa cells prelabelled with (/sup 3/H) inositol. Analysis by high performance anion exchange chromatography of the inositol-phosphate compounds revealed the existence of two additional inositol tetrakisphosphate (InsP4) isomers in proximity to Ins-1,3,4,5-P4, the known phosphorylation product of Ins-1,4,5-trisphosphate and precursor of Ins-1,3,4-trisphosphate. Both of these new compounds showed a slow increase after stimulation with angiotensin II. The structure of one of these new InsP4 isomers, which is a phosphorylation product of Ins-1,3,4-P3, was deduced by its resistance to periodate oxidation to be Ins-1,3,4,6-P4. The existence of multiple cycles of phosphorylation-dephosphorylation reactions for the processing of Ins-1,4,5-P4 may represent a new aspect of the inositol-lipid related signalling mechanism in agonist-activated target cells.
- Research Organization:
- National Institute of Child Health and Human Development, Bethesda, MD
- OSTI ID:
- 5630474
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Vol. 148:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANGIOTENSIN
BIOCHEMICAL REACTION KINETICS
PHOSPHOLIPIDS
BIOSYNTHESIS
ADRENAL GLANDS
CATTLE
INOSITOL
PHOSPHORYLATION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
BODY
CARBOHYDRATES
CARDIOVASCULAR AGENTS
CHEMICAL REACTIONS
DOMESTIC ANIMALS
DRUGS
ENDOCRINE GLANDS
ESTERS
GLANDS
GLOBULINS
INOSITOLS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPIDS
MAMMALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PROTEINS
REACTION KINETICS
RUMINANTS
SACCHARIDES
SYNTHESIS
VASOCONSTRICTORS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques