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Title: Estrogen- and progestin-receptor complexes and their interaction with rat liver cell nuclei during ontogenesis

Steroid-receptor complexes (SRC) of estrogens and progestins have been isolated from the rat liver and purified 1500-2000-fold. Both in the state of the initial cytosol and purified 2000-fold, the SRC were characterized by gel filtration on Sephadex G-100 and by DEAE-cellulose chromatography. The purified SRC from the rat liver were used for binding to isolated liver cell nuclei from rats of different ages (1.5 months, 6 months, 12 months, 24 months). The maximum binding of SRC of progestins and estrogens from the rat liver is observed with homologous nuclei of 1.5-month-old rats. With age the binding of SRC by the nuclei decreases progressively and reaches a minimum by 24 months. The detected differences in the binding of SRC by the nuclei of cells of animals of different ages, in their opinion, may lie at the basis of changes in the functioning of the organism under the influence of hormones at different stages of ontogenesis.
Authors:
; ; ;
Publication Date:
OSTI Identifier:
5629373
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemistry (Engl. Transl.); (United States); Journal Volume: 51:5; Other Information: Translated from Biokhimiya; 51: 5, 775-781(May 1986)
Research Org:
Sector of Gerontology, Minsk, USSR
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ESTROGENS; RECEPTORS; LIVER CELLS; CELL NUCLEI; ONTOGENESIS; RADIORECEPTOR ASSAY; PROGESTERONE; STEROIDS; AGE DEPENDENCE; BINDING ENERGY; CHROMATOGRAPHY; COMPLEXES; LABELLING; LIVER; RADIOACTIVITY; RATS; SCINTILLATION COUNTING; TRITIUM COMPOUNDS; ANIMAL CELLS; ANIMALS; BODY; CELL CONSTITUENTS; COUNTING TECHNIQUES; DIGESTIVE SYSTEM; ENERGY; GLANDS; HORMONES; ISOTOPE APPLICATIONS; KETONES; LABELLED COMPOUNDS; MAMMALS; MEMBRANE PROTEINS; ORGANIC COMPOUNDS; ORGANS; PREGNANES; PROTEINS; RODENTS; SEPARATION PROCESSES; SOMATIC CELLS; STEROID HORMONES; TRACER TECHNIQUES; VERTEBRATES 550301* -- Cytology-- Tracer Techniques; 550201 -- Biochemistry-- Tracer Techniques