Isoelectric focusing of wound-induced tomato ACC synthase
Abstract
Several techniques of electrofocusing have been used to determine whether 1-aminocyclopropane-1-carboxylate (ACC) synthase isolated from wounded tomato pericarp tissue exists in different isoforms, each with its characteristic isoelectric point (pI). The pI of the native enzyme was found to be 6.0 {plus minus} 0.2. When radiolabeled, denatured ACC synthase was electrofocused by non-equilibrium pH gradient electrophoresis (NEpHGE), the enzyme separated into four discernible spots which, upon reaching equilibrium, ranged in pI from 6.6 to 6.9. Immunopurified ACC synthase from four tomato cultivars (Duke, Cornell, Mountain Pride and Pik Red) migrated in each case as a 50-kDa protein on sodium dodecyl sulfate polyacrylamide gels (SDS-PAGE). We propose that native ACC synthase in extracts of tomato pericarp tissue exists in one single form and that the charge heterogeneities observed upon electrofocusing of denatured enzyme result from modifications of preexisting protein.
- Authors:
-
- Michigan State Univ., East Lansing (USA)
- Publication Date:
- OSTI Identifier:
- 5629233
- Report Number(s):
- CONF-9007196-
Journal ID: ISSN 0079-2241; CODEN: PPYSA
- Resource Type:
- Conference
- Journal Name:
- Plant Physiology, Supplement; (USA)
- Additional Journal Information:
- Journal Volume: 93:1; Conference: Annual meeting of the American Society of Plant Physiologists, Indianapolis, IN (USA), 29 Jul - 2 Aug 1990; Journal ID: ISSN 0079-2241
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; TOMATOES; BIOLOGICAL STRESS; CARBOXYLATION; ENZYMES; PROTEINS; CHEMICAL REACTIONS; FOOD; FRUITS; ORGANIC COMPOUNDS; 550100* - Behavioral Biology
Citation Formats
White, J A, and Kende, H. Isoelectric focusing of wound-induced tomato ACC synthase. United States: N. p., 1990.
Web.
White, J A, & Kende, H. Isoelectric focusing of wound-induced tomato ACC synthase. United States.
White, J A, and Kende, H. 1990.
"Isoelectric focusing of wound-induced tomato ACC synthase". United States.
@article{osti_5629233,
title = {Isoelectric focusing of wound-induced tomato ACC synthase},
author = {White, J A and Kende, H},
abstractNote = {Several techniques of electrofocusing have been used to determine whether 1-aminocyclopropane-1-carboxylate (ACC) synthase isolated from wounded tomato pericarp tissue exists in different isoforms, each with its characteristic isoelectric point (pI). The pI of the native enzyme was found to be 6.0 {plus minus} 0.2. When radiolabeled, denatured ACC synthase was electrofocused by non-equilibrium pH gradient electrophoresis (NEpHGE), the enzyme separated into four discernible spots which, upon reaching equilibrium, ranged in pI from 6.6 to 6.9. Immunopurified ACC synthase from four tomato cultivars (Duke, Cornell, Mountain Pride and Pik Red) migrated in each case as a 50-kDa protein on sodium dodecyl sulfate polyacrylamide gels (SDS-PAGE). We propose that native ACC synthase in extracts of tomato pericarp tissue exists in one single form and that the charge heterogeneities observed upon electrofocusing of denatured enzyme result from modifications of preexisting protein.},
doi = {},
url = {https://www.osti.gov/biblio/5629233},
journal = {Plant Physiology, Supplement; (USA)},
issn = {0079-2241},
number = ,
volume = 93:1,
place = {United States},
year = {Tue May 01 00:00:00 EDT 1990},
month = {Tue May 01 00:00:00 EDT 1990}
}