Synthesis and processing in Escherichia coli of human leucocyte interferon fused with the signal sequence of Bacillus amyloliquefaciens a-amylase

Journal Article · Fri Nov 01 00:00:00 EST 1985 · Dokl. Biol. Sci. (Engl. Transl.); (United States)

OSTI ID:5629013

Sorokin, A V; Avakov, A S; Bogush, V G; Kostrov, S V; Gaiga, G Z; Iomantas, Yu V; Abalakina, E G; Stepanov, A I; Strongin, A Ya; Kozlov, Yu I; Debabov, V G

Earlier, the authors reported cloning of the alpha-amylase gene of B. amyloliquefaciens in B. subtilis and E. coli. Currently, the authors report results on the expression of the hybrid gene consisting of the DNA fragment coding for the leader part of B. amyloliquefaciens alpha-amylase and the structural part of the human interferon alpha-2 in E. coli cells. This gene contains an additional methionine codon at the 5'-terminal, which codes for the interferon structure (without its own signal peptide). The interferon gene was inserted into plasmid /sub p/TG 278 at the cleavage site of EcoRI. The structure of the plasmid thus obtained the signal peptide of amylase, five amino acids (Val-Gly-Glu-Phe-Met), and the structural part of the interferon. The E. coli C600 cells carrying plasmid pTGA6 were used to study interferon secretions. The interferon activity was determined radioimmunologically with the use of monoclonal anti-bodies NK2.

• Research Organization: All-Union Scientific-Research Institute of Genetics and Breeding of Industrial Microorganisms, Moscow, USSR
• OSTI ID: 5629013
• Journal Information: Dokl. Biol. Sci. (Engl. Transl.); (United States), Vol. 282:1-6; Other Information: Translated from Dokl. Akad. Nauk SSSR; 282: No. 2, 1490-1493(Jun 1985)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
INTERFERON
AMINO ACID SEQUENCE
BIOLOGICAL EFFECTS
BIOSYNTHESIS
GENE RECOMBINATION
MONOCLONAL ANTIBODIES
RADIOIMMUNOASSAY
ALKALINE PHOSPHATASE
AMYLASE
BACILLUS
CODONS
DNA
DNA SEQUENCING
ESCHERICHIA COLI
GENETIC MAPPING
LEUKOCYTES
MAN
PLASMIDS
ANIMALS
ANTIBODIES
BACTERIA
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
ENZYMES
ESTERASES
GLYCOSYL HYDROLASES
GROWTH FACTORS
HYDROLASES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LYMPHOKINES
MAMMALS
MAPPING
MATERIALS
MICROORGANISMS
MITOGENS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PHOSPHATASES
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNOLOGY
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES
550401* - Genetics- Tracer Techniques