Gel permeation chromatography of crystalline cellulose from the secondary wall of intact cotton fibers
- Texas A M Univ., College Station (USA)
({sup 14}C)glucose or UDP-({sup 14}C)-glucose incorporation into polysaccharides in cotton fiber during secondary wall formation predominantly labels {beta} 1,3- and {beta} 1,4-glucan. The amount of radioactivity in the individual {beta}-glucans was determined by analyzing the partially methylated alditol acetates from the ({sup 14}C) glucans before and after treatment with Updegraff's acetic-nitric reagent. Hot acetic-nitric hydrolyzes {beta} 1,3-glucan leaving resistant crystalline cellulose. In this research we have determined the mol wt characteristics of the crystalline cellulose polymer synthesized from ({sup 14}C) glucose in intact cotton fibers. The ({sup 14}C)-crystalline cellulose in the secondary wall was isolated using the acetic-nitric reagent, dissolved in a non-degrading solvent of lithium chloride/N,N-dimethylacetamide and separated on columns of Ultrastyragel by gel permeation chromatography. The ({sup 14}C)-crystalline cellulose separates into individual cellulose chains with mol wts of 10{sup 7} to 10{sup 4}. The weight average mol wt (Mw) of the polymer is 710,000. The distribution of the chains within the polymer approximates a normal distribution with 95% of the chains distributed with {plus minus} 2 std dev of the mean typical of other biopolymers.
- OSTI ID:
- 5628365
- Report Number(s):
- CONF-9007196-; CODEN: PPYSA
- Journal Information:
- Plant Physiology, Supplement; (USA), Vol. 93:1; Conference: Annual meeting of the American Society of Plant Physiologists, Indianapolis, IN (USA), 29 Jul - 2 Aug 1990; ISSN 0079-2241
- Country of Publication:
- United States
- Language:
- English
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