Phosphatidylethanolamine turnover is an early event in the response of NB sub 2 lymphoma cells to prolactin
- St. Jude Children's Research Hospital, Memphis, TN (United States)
The effect of prolactin on phospholipid metabolism in the prolactin-dependent rat lymphoma cell line Nb{sub 2} was investigated in cells prelabeled with ({sup 3}H)arachidonic acid or ({sup 3}H)ethanolamine. Prolactin (20 ng/ml) caused (a) a 20-60% loss of radiolabeled phosphatidylethanolamine within 0.5 to 2 min, (b) a loss of ({sup 3}H)ethanolamine-labeled phosphatidylethanolamine from crude membranes, (c) a rapid accumulation of ({sup 3}H) phosphoethanolamine and ({sup 3}H)ethanolamine, and (d) a transient increase (15 s to 2 min) in prostaglandin F{sub 2}{alpha}, and E{sub 2}. Arachidonic acid (1-2 {mu}g/ml) induced Nb{sub 2} cell growth but prostaglandin F{sub 2}{alpha}, E{sub 2}, ethanolamine, and phosphoethanolamine did not. Prostaglandin E{sub 2} inhibited while prostaglandin F{sub 2}{alpha} enhanced growth in the presence of prolactin or arachidonic acid. These results suggest that stimulation of Nb{sub 2} cell growth by prolactin linked to activation of a phosphatidylethanolamine-specific phospholipase C. Arachidonic acid and prostaglandin F{sub 2}{alpha} may participate in regulating the mitogenic actin of prolactin.
- OSTI ID:
- 5623016
- Journal Information:
- Experimental Cell Research; (United States), Vol. 184:1; ISSN 0014-4827
- Country of Publication:
- United States
- Language:
- English
Similar Records
Activation of phospholipase A/sub 2/ by carbon tetrachloride in isolated rat hepatocytes
Nonenzymatic glycation of phosphatidylethanolamine in erythrocyte vesicles
Related Subjects
LH
BIOLOGICAL EFFECTS
PHOSPHOLIPIDS
METABOLISM
TRITIUM COMPOUNDS
THIN-LAYER CHROMATOGRAPHY
AMINES
ARACHIDONIC ACID
LYMPHOMAS
PROSTAGLANDINS
TUMOR CELLS
ANIMAL CELLS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DISEASES
ESTERS
GONADOTROPINS
HORMONES
HYDROGEN COMPOUNDS
IMMUNE SYSTEM DISEASES
LIPIDS
MONOCARBOXYLIC ACIDS
NEOPLASMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
SEPARATION PROCESSES
550301* - Cytology- Tracer Techniques