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Title: Metabolism of benzonitrile and butyronitrile by Klebsiella pneumoniae

Abstract

A strain of Klebsiella pneumoniae that used aliphatic nitriles as the sole source of nitrogen was adapted to benzonitrile as the sole source of carbon and nitrogen. Gas chromatographic and mass spectral analyses of culture filtrates indicated that K. pneumoniae metabolized 8.4 mM benzonitrile to 4.0 mM benzoic acid and 2.7 mM ammonia. In addition, butyronitrile was metabolized to butyramide and ammonia. The isolate also degraded mixtures of benzonitrile and aliphatic nitriles. Cell extracts contained nitrile hydratase and amidase activities. The enzyme activities were higher with butyronitrile and butyramide than with benzonitrile and benzamide, and amidase activities were twofold higher than nitrile hydratase activities. K. pneumoniae appears promising for the bioremediation of sites contaminated with aliphatic and aromatic nitriles.

Authors:
; ;  [1]
  1. Food and Drug Administration, Jefferson, AK (United States)
Publication Date:
OSTI Identifier:
5617109
Resource Type:
Journal Article
Journal Name:
Applied and Environmental Microbiology; (United States)
Additional Journal Information:
Journal Volume: 58:1; Journal ID: ISSN 0099-2240
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; KLEBSIELLA; METABOLISM; NITRILES; BIODEGRADATION; AMMONIA; BENZOIC ACID; BIOLOGICAL PATHWAYS; ENZYME ACTIVITY; MIXTURES; BACTERIA; CARBOXYLIC ACIDS; CHEMICAL REACTIONS; DECOMPOSITION; DISPERSIONS; HYDRIDES; HYDROGEN COMPOUNDS; MICROORGANISMS; MONOCARBOXYLIC ACIDS; NITROGEN COMPOUNDS; NITROGEN HYDRIDES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; 550500* - Metabolism

Citation Formats

Nawaz, M S, Heinze, T M, and Cerniglia, C E. Metabolism of benzonitrile and butyronitrile by Klebsiella pneumoniae. United States: N. p., 1992. Web.
Nawaz, M S, Heinze, T M, & Cerniglia, C E. Metabolism of benzonitrile and butyronitrile by Klebsiella pneumoniae. United States.
Nawaz, M S, Heinze, T M, and Cerniglia, C E. 1992. "Metabolism of benzonitrile and butyronitrile by Klebsiella pneumoniae". United States.
@article{osti_5617109,
title = {Metabolism of benzonitrile and butyronitrile by Klebsiella pneumoniae},
author = {Nawaz, M S and Heinze, T M and Cerniglia, C E},
abstractNote = {A strain of Klebsiella pneumoniae that used aliphatic nitriles as the sole source of nitrogen was adapted to benzonitrile as the sole source of carbon and nitrogen. Gas chromatographic and mass spectral analyses of culture filtrates indicated that K. pneumoniae metabolized 8.4 mM benzonitrile to 4.0 mM benzoic acid and 2.7 mM ammonia. In addition, butyronitrile was metabolized to butyramide and ammonia. The isolate also degraded mixtures of benzonitrile and aliphatic nitriles. Cell extracts contained nitrile hydratase and amidase activities. The enzyme activities were higher with butyronitrile and butyramide than with benzonitrile and benzamide, and amidase activities were twofold higher than nitrile hydratase activities. K. pneumoniae appears promising for the bioremediation of sites contaminated with aliphatic and aromatic nitriles.},
doi = {},
url = {https://www.osti.gov/biblio/5617109}, journal = {Applied and Environmental Microbiology; (United States)},
issn = {0099-2240},
number = ,
volume = 58:1,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1992},
month = {Wed Jan 01 00:00:00 EST 1992}
}