Monitoring of individual human exposure to aflatoxins (AF) and N-nitrosamines (NNO) by immunoassays
Abstract
Highly sensitive immunoassays have been used to quantitate aflatoxins (AF) and N-nitrosamines (NNO) in human body fluids and tissues, respectively. This approach was taken in order to quantitate environmental exposure to these agents at an individual level to facilitate the investigation of their role in the etiology of human cancer. In order to analyse AF in human urine, an immunopurification step has been developed by using AF-specific antibody bound to AH-Sepharose 4B gel in a small (4-ml gel volume) affinity column prior to enzyme-linked immunosorbent assay (ELISA). The ELISA can be used to quantitate aflatoxin B1 (AFB1) over the range 0.01 ng/ml to 10 ng/ml and the assay system has been validated by using human urine samples spiked with AFB1 over this concentration range. In addition, 29 urine samples from the Philippines have been analyzed and found to contain a range of levels from zero to 4.25 ng/ml AFB1 equivalent with a mean of 0.875 ng/ml. This compared with a mean of 0.066 ng/ml AFB1 equivalent in samples from France. Radioimmunoassay of O6-methyldeoxyguanosine (O6-medG) has been performed on human esophageal and cardiac stomach mucosal DNA from tissue samples obtained during surgery in Linxian County, People's Republic of China, an areamore »
- Authors:
- Publication Date:
- Research Org.:
- International Agency for Research on Cancer, Lyon, France
- OSTI Identifier:
- 5616563
- Resource Type:
- Journal Article
- Journal Name:
- J. Cell. Biochem.; (United States)
- Additional Journal Information:
- Journal Volume: 2
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; AFLATOXIN; CARCINOGEN SCREENING; ESOPHAGUS; NEOPLASMS; ETIOLOGY; NITROSAMINES; STOMACH; BODY FLUIDS; CARCINOGENS; CHINA; COMPARATIVE EVALUATIONS; DNA; DNA REPAIR; ENVIRONMENTAL EXPOSURE; FRANCE; PHILIPPINES; RADIOIMMUNOASSAY; URINE; AMINES; ANTIGENS; ASIA; BIOLOGICAL MATERIALS; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; BIOLOGICAL WASTES; BODY; DEVELOPING COUNTRIES; DIGESTIVE SYSTEM; DISEASES; EUROPE; GASTROINTESTINAL TRACT; IMMUNOASSAY; IMMUNOLOGY; ISLANDS; ISOTOPE APPLICATIONS; MATERIALS; NITROSO COMPOUNDS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; RADIOASSAY; RADIOIMMUNOLOGY; RECOVERY; REPAIR; SCREENING; TOXIC MATERIALS; TOXINS; TRACER TECHNIQUES; WASTES; WESTERN EUROPE; 560306* - Chemicals Metabolism & Toxicology- Man- (-1987); 550901 - Pathology- Tracer Techniques
Citation Formats
Wild, C P, Umbenhauer, D, Chapot, B, and Montesano, R. Monitoring of individual human exposure to aflatoxins (AF) and N-nitrosamines (NNO) by immunoassays. United States: N. p., 1986.
Web. doi:10.1002/jcb.240300206.
Wild, C P, Umbenhauer, D, Chapot, B, & Montesano, R. Monitoring of individual human exposure to aflatoxins (AF) and N-nitrosamines (NNO) by immunoassays. United States. https://doi.org/10.1002/jcb.240300206
Wild, C P, Umbenhauer, D, Chapot, B, and Montesano, R. 1986.
"Monitoring of individual human exposure to aflatoxins (AF) and N-nitrosamines (NNO) by immunoassays". United States. https://doi.org/10.1002/jcb.240300206.
@article{osti_5616563,
title = {Monitoring of individual human exposure to aflatoxins (AF) and N-nitrosamines (NNO) by immunoassays},
author = {Wild, C P and Umbenhauer, D and Chapot, B and Montesano, R},
abstractNote = {Highly sensitive immunoassays have been used to quantitate aflatoxins (AF) and N-nitrosamines (NNO) in human body fluids and tissues, respectively. This approach was taken in order to quantitate environmental exposure to these agents at an individual level to facilitate the investigation of their role in the etiology of human cancer. In order to analyse AF in human urine, an immunopurification step has been developed by using AF-specific antibody bound to AH-Sepharose 4B gel in a small (4-ml gel volume) affinity column prior to enzyme-linked immunosorbent assay (ELISA). The ELISA can be used to quantitate aflatoxin B1 (AFB1) over the range 0.01 ng/ml to 10 ng/ml and the assay system has been validated by using human urine samples spiked with AFB1 over this concentration range. In addition, 29 urine samples from the Philippines have been analyzed and found to contain a range of levels from zero to 4.25 ng/ml AFB1 equivalent with a mean of 0.875 ng/ml. This compared with a mean of 0.066 ng/ml AFB1 equivalent in samples from France. Radioimmunoassay of O6-methyldeoxyguanosine (O6-medG) has been performed on human esophageal and cardiac stomach mucosal DNA from tissue samples obtained during surgery in Linxian County, People's Republic of China, an area of high risk for both esophageal and stomach cancer. Using the methodology described and having 1 mg of hydrolyzed DNA allows the detection of approximately 25 fmol O6medG per mg DNA.},
doi = {10.1002/jcb.240300206},
url = {https://www.osti.gov/biblio/5616563},
journal = {J. Cell. Biochem.; (United States)},
number = ,
volume = 2,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1986},
month = {Wed Jan 01 00:00:00 EST 1986}
}