Double labeling of human leukemic cells using /sup 3/H-cytarabine and monoclonal antibody against bromodeoxyuridine

Journal Article · Fri Feb 01 00:00:00 EST 1985 · Cancer Treat. Rep.; (United States)

OSTI ID:5612164

Raza, A; Preisler, H D

A new technique using immunofluorescence and autoradiography is described, in which the DNA of cells in S phase are labeled with two different probes. This method makes it possible to study the relationship between DNA synthesis and the uptake and/or incorporation of chemotherapeutic agents into normal or neoplastic cells. An example is provided in which the incorporation of /sup 3/H-cytarabine into DNA is demonstrated to occur only in cells which were synthesizing DNA during exposure to /sup 3/H-cytarabine. Other radioactively labeled probes can be used as well.

• Research Organization: Roswell Park Memorial Institute, Buffalo, NY
• OSTI ID: 5612164
• Journal Information: Cancer Treat. Rep.; (United States), Vol. 69:2
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ANTINEOPLASTIC DRUGS
UPTAKE
DNA
DOUBLE LABELLING
TUMOR CELLS
AUTORADIOGRAPHY
BUDR
CELL CULTURES
CELL CYCLE
CHEMOTHERAPY
DIAGNOSTIC USES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL CELLS
ANTIMETABOLITES
AZINES
BROMOURACILS
DRUGS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RIBOSIDES
THERAPY
URACILS
USES
550601* - Medicine- Unsealed Radionuclides in Diagnostics