Molecular analysis of chromosomal rearrangements using pulsed field gel electrophoresis and somatic cell hybrids
- Florida Inst. of Tech., Melbourne (United States)
Many human genetic diseases, including some cancers, are characterized by consistent chromosome abnormalities, such as deletions and translocations. Analyses of these mutations often prove crucial to the eventual cloning and characterization of the gene(s) responsible for the disease. Two methods for analyzing these chromosome abnormalities have been developed in recent years: somatic cell hybridization and pulsed field gel electrophoresis (PFGE). Somatic cell hybridization is a technique for segregating an aberrant chromosome from its normal homologue in a cell derived from an unrelated species, which is usually a rodent. Demonstrations of these analytic techniques are presented, using as an example chromosomal abnormalities involving human chromosome band 11p13, the locus for the Wilms' tumor, aniridia, genitourinary abnormality, and mental retardation (WAGR) syndrome.
- OSTI ID:
- 5610651
- Journal Information:
- Environmental and Molecular Mutagenesis; (United States), Vol. 18:4; ISSN 0893-6692
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHROMOSOMAL ABERRATIONS
GENETIC MAPPING
HEREDITARY DISEASES
MOLECULAR BIOLOGY
BIOLOGICAL MARKERS
DNA-CLONING
ELECTROPHORESIS
HETEROCHROMOSOMES
KIDNEYS
UROGENITAL SYSTEM DISEASES
BODY
CHROMOSOMES
CLONING
DISEASES
DNA HYBRIDIZATION
HYBRIDIZATION
MAPPING
MUTATIONS
ORGANS
560300* - Chemicals Metabolism & Toxicology