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Title: Radiation inactivation (target size analysis) of the gonadotropin-releasing hormone receptor: evidence for a high molecular weight complex

Abstract

In the present study we used radiation inactivation (target size analysis) to measure the functional mol wt of the GnRH receptor while it is still a component of the plasma membrane. This technique is based on the observation that an inverse relationship exists between the dose-dependent inactivation of a macromolecule by ionizing radiation and the size of that macromolecule. This method demonstrates a mol wt of 136,346 +/- 8120 for the GnRH receptor. This estimate is approximately twice that obtained (60,000) by photoaffinity labeling with a radioactive GnRH analog followed by electrophoresis under denaturing conditions and, accordingly, presents the possibility that the functional receptor consists of a high mol wt complex in its native state. The present studies indicate that the GnRH receptor is either a single weight class of protein or several closely related weight classes, such as might occur due to protein glycosylation.

Authors:
;
Publication Date:
Research Org.:
Duke Univ. Medical Center, Durham, NC
OSTI Identifier:
5609010
Resource Type:
Journal Article
Journal Name:
Endocrinology; (United States)
Additional Journal Information:
Journal Volume: 116:4
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CELL MEMBRANES; BIOLOGICAL RADIATION EFFECTS; HORMONES; RECEPTORS; MOLECULAR WEIGHT; FEMALES; HYPOTHALAMUS; PITUITARY GLAND; RATS; ANIMALS; BIOLOGICAL EFFECTS; BODY; BRAIN; CELL CONSTITUENTS; CENTRAL NERVOUS SYSTEM; ENDOCRINE GLANDS; GLANDS; MAMMALS; MEMBRANES; NERVOUS SYSTEM; ORGANS; RADIATION EFFECTS; RODENTS; VERTEBRATES; 560121* - Radiation Effects on Cells- External Source- (-1987)

Citation Formats

Conn, P M, and Venter, J C. Radiation inactivation (target size analysis) of the gonadotropin-releasing hormone receptor: evidence for a high molecular weight complex. United States: N. p., 1985. Web. doi:10.1210/endo-116-4-1324.
Conn, P M, & Venter, J C. Radiation inactivation (target size analysis) of the gonadotropin-releasing hormone receptor: evidence for a high molecular weight complex. United States. https://doi.org/10.1210/endo-116-4-1324
Conn, P M, and Venter, J C. 1985. "Radiation inactivation (target size analysis) of the gonadotropin-releasing hormone receptor: evidence for a high molecular weight complex". United States. https://doi.org/10.1210/endo-116-4-1324.
@article{osti_5609010,
title = {Radiation inactivation (target size analysis) of the gonadotropin-releasing hormone receptor: evidence for a high molecular weight complex},
author = {Conn, P M and Venter, J C},
abstractNote = {In the present study we used radiation inactivation (target size analysis) to measure the functional mol wt of the GnRH receptor while it is still a component of the plasma membrane. This technique is based on the observation that an inverse relationship exists between the dose-dependent inactivation of a macromolecule by ionizing radiation and the size of that macromolecule. This method demonstrates a mol wt of 136,346 +/- 8120 for the GnRH receptor. This estimate is approximately twice that obtained (60,000) by photoaffinity labeling with a radioactive GnRH analog followed by electrophoresis under denaturing conditions and, accordingly, presents the possibility that the functional receptor consists of a high mol wt complex in its native state. The present studies indicate that the GnRH receptor is either a single weight class of protein or several closely related weight classes, such as might occur due to protein glycosylation.},
doi = {10.1210/endo-116-4-1324},
url = {https://www.osti.gov/biblio/5609010}, journal = {Endocrinology; (United States)},
number = ,
volume = 116:4,
place = {United States},
year = {Mon Apr 01 00:00:00 EST 1985},
month = {Mon Apr 01 00:00:00 EST 1985}
}