Clonality in myeloproliferative disorders: Analysis by means of polymerase chain reaction

Journal Article · Thu Aug 01 00:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.15.6848· OSTI ID:5604292

Gilliland, D G; Blanchard, K L; Levy, J; Perrin, S; Bunn, H F ^[1]

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1. Harvard Medical School, Boston, MA (United States)

The myeloproliferative syndromes are acquired disorders of hematopoiesis that provide insights into the transition from somatic cell mutation to neoplasia. The clonal origin of specific blood cells can be assessed in patients with X chromosome-linked polymorphisms, taking advantage of random inactivation of the X chromosome. The authors have adapted the PCR for determination of clonality on as few as 100 cells, including individual colonies grown in culture. Amplifying a polymorphic portion of the X chromosome-linked phosphoglycerate kinase (PGK) gene after selective digestion of the active X chromosome with a methylation-sensitive restriction enzyme gave results fully concordant with standard Southern blotting of DNA samples form normal (polyclonal) polymorphonuclear cells (PMN) as well as clonal PMN from patients with myelodysplastic syndrome and polycythemia vera (PCV). They have used this technique to demonstrate heterogeneity of lineage involvement in patients with PCV. The same clinical phenotype may arise from clonal proliferation of different hematopoietic progenitors.

• OSTI ID: 5604292
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:15; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CLONE CELLS
CYTOLOGICAL TECHNIQUES
HEREDITARY DISEASES
MOLECULAR BIOLOGY
HUMAN X CHROMOSOME
DNA SEQUENCING
ANEMIAS
CARCINOGENESIS
DNA POLYMERASES
HEMATOPOIETIC SYSTEM
PHENOTYPE
PHOSPHORUS 32
RFLPS
SOMATIC MUTATIONS
WOMEN
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CELL CULTURES
CHROMOSOMES
DAYS LIVING RADIOISOTOPES
DISEASES
ENZYMES
FEMALES
HEMIC DISEASES
HETEROCHROMOSOMES
HUMAN CHROMOSOMES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MUTATIONS
NUCLEI
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PATHOGENESIS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PRIMATES
PROTEINS
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
SYMPTOMS
TRANSFERASES
VERTEBRATES
X CHROMOSOME
550200* - Biochemistry