Synthesis and evaluation of radioactive and fluorescent residualizing labels for monitoring protein degradation in vivo and in vitro
Residualizing labels for proteins, such as dilactitol-{sup 125}I-tyramine, are tracers which have been used to identify the tissue and cellular sites of catabolism of long-lived plasma proteins, such as albumin. The radioactive degradation products formed from labeled proteins are relatively large and hydrophilic. These tracers accumulate in lysosomes following uptake and catabolism of the carrier protein. However, the gradual loss of the catabolites from cells has limited the usefulness of these radioactive labels in studies on longer-lived proteins. The objective of this dissertation was to design a radioactive residualizing label, Inulin-{sup 125}I-tyramine ({sup 125}I-InTn), that would be retained more efficiently in cells than existing labels and to develop and evaluate the first fluorescent residualizing label, N,N-dilactitol-N{prime}-fluoresceinyl-ethylenediamine (DLF).
- Research Organization:
- South Carolina Univ., Columbia, SC (USA)
- OSTI ID:
- 5601003
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PROTEINS
LABELLING
RADIOPHARMACEUTICALS
CHEMICAL PREPARATION
CATABOLISM
EVALUATION
FLUORESCENCE
IODINE 125
LYSOSOMES
TYRAMINE
AMINES
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LUMINESCENCE
METABOLISM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
PHENOLS
RADIOISOTOPES
SYMPATHOMIMETICS
SYNTHESIS
550201* - Biochemistry- Tracer Techniques