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Title: Radiation-induced changes in the cuticular hydrocarbons of the granary weevil and their relationship to desiccation and adult mortality

Radiation from the nuclear waste products, such as Cesium-137, offers a scope and could be used for large scale disinfestation of grain. It is known that 0.15 to 0.20 kGy dose of gamma radiation is sufficient to kill insects in grain and grain products. However, the mode of action (in terms of lethal effects) is not understood. The purpose of this project, therefore, is to study the ways in which gamma radiation causes death in the granary weevil. Sitophilus granarius (L.) is a major and cosmopolitan pest of stored grain all over the world. Radiation damage, in particular the specific effects on the physiology of the insects exposed to radiation has been elucidated. In stored grain insects, conservation of water is a critical factor for their survival. Epicuticular hydrocarbons play an important role in water proofing. The laboratory rearing of the granary weevil was standardized so that large numbers of weevils of known ages could be produced for experimentation. Stock cultures were maintained at 27 {plus minus} 2{degree}C and 65 {plus minus} 5% R.H. Tests with various age groups (adults) and different doses of gamma radiation indicate that lethal effects are both age and dose related. Younger weevils, in general,more » survive for a longer period after irradiation compared to older weevils. Complete mortality results within about two weeks after exposure to gamma radiation at dose of 0.15 kGy or above. Data on wet and dry weights of the weevils kept at different (low, medium and higher) levels of humidity after irradiation indicate that gamma radiation induces greater water loss leading to desiccation and early death. Low humidity environment (17% R.H.) greatly accelerates lethal effects.« less
Authors:
 [1]
  1. (Selma Univ., AL (USA). Div. of Natural and Applied Sciences)
Publication Date:
OSTI Identifier:
5596445
Report Number(s):
DOE/CH/10288-T4
ON: DE89015540
DOE Contract Number:
FG02-86CH10288
Resource Type:
Technical Report
Research Org:
Selma Univ., AL (USA). Div. of Natural and Applied Sciences
Sponsoring Org:
DOE/DP
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; BEETLES; MORTALITY; EXOSKELETON; CHEMICAL COMPOSITION; DOSE-RESPONSE RELATIONSHIPS; GAMMA RADIATION; GAS CHROMATOGRAPHY; GRAIN DISINFESTATION; HUMIDITY; LETHAL IRRADIATION; LIFE SPAN; MODIFICATIONS; PEST CONTROL; POST-IRRADIATION EXAMINATION; PROGRESS REPORT; RADIOINDUCTION; RADIOPRESERVATION; RADIOSENSITIVITY; SURVIVAL TIME; TEMPERATURE DEPENDENCE; WATER REQUIREMENTS; ANIMALS; ARTHROPODS; BODY; CHROMATOGRAPHY; COLEOPTERA; CONTROL; DISINFESTATION; DOCUMENT TYPES; ELECTROMAGNETIC RADIATION; INSECTS; INVERTEBRATES; IONIZING RADIATIONS; IRRADIATION; ORGANS; PRESERVATION; RADIATIONS; SEPARATION PROCESSES; SKELETON 553003* -- Agriculture & Food Technology-- Pest & Disease Control-- (1987-); 560152 -- Radiation Effects on Animals-- Animals