New immobilized cell system with protection against toxic solvents

Journal Article · Thu Jan 01 00:00:00 EST 1987 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260300105· OSTI ID:5595398

Tanaka, H; Harada, S; Kurosawa, H; Yajima, M

A new immobilized cell system providing protection against toxic solvents was investigated so that normal fermentations could be carried out in a medium containing toxic solvents. The system consists of immobilized growing cells in Ca-alginate gel beads to which vegetable oils, which are inexpensive absorbents of solvents, had been added. The ethanol fermentation of Saccharomyces cerevisiae ATCC 26603 was used as a model fermentation to study the protection afforded by the system against solvent toxicities. The fermentation was inhibited by solvents such as 2-octanol, benzene, toluene, and phenol. Ethanol production of one batch was not finished even after 35 h using immobilized growing yeast cells in conventional Ca-alginate gel beads in an ethanol production medium (5% glucose) containing 0.1% 2-octanol, which is used as a solvent for liquid-liquid extraction and is one of the most toxic solvents in our experiments. With the new immobilized growing cell system using vegetable oils, however, four repeated batch fermentations were completed in 35 h. Castor oil provided even more protection than soy bean, olive, and tung oils, and it was possible to complete six repeated batches in 35 h. The immobilized cell system with vegetable oils also provided protection against other toxic solvents such as benzene and toluene. A possible mechanism for the protective function of the new immobilized cell system is discussed.

• Research Organization: Univ. of Tsukuba, Ibaraki, Japan
• OSTI ID: 5595398
• Journal Information: Biotechnol. Bioeng.; (United States), Vol. 30:1
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BENZENE
TOXICITY
ETHANOL
YIELDS
GLUCOSE
FERMENTATION
OCTANOLS
PHENOL
SACCHAROMYCES CEREVISIAE
IMMOBILIZED CELLS
TOLUENE
ALCOHOLS
ALDEHYDES
ALKYLATED AROMATICS
AROMATICS
BIOCONVERSION
CARBOHYDRATES
FUNGI
HEXOSES
HYDROCARBONS
HYDROXY COMPOUNDS
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PHENOLS
PLANTS
SACCHARIDES
SACCHAROMYCES
YEASTS
090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989)
560300 - Chemicals Metabolism & Toxicology