Increase of O6-methylguanine-DNA-methyltransferase and N3-methyladenine glycosylase RNA transcripts in rat hepatoma cells treated with DNA-damaging agents

Journal Article · Wed May 15 00:00:00 EDT 1991 · Biochemical and Biophysical Research Communications; (United States)

DOI:https://doi.org/10.1016/0006-291X(91)90395-N· OSTI ID:5592581

Laval, F ^[1]

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1. U247 INSERM, Institut Gustave Roussy, Villejuif (France)

A variety of DNA-damaging agents increase the O6-methylguanine-DNA-methyltransferase (transferase) and the N3-methyladenine (3-meAde)-DNA-glycosylase activities in a rat hepatoma cell line (H4 cells). Using two cDNA expressing either the rat 3-meAde-DNA-glycosylase or the transferase, the level of mRNA transcripts was measured by hybridization in H4 cells treated with three different inducing agents, gamma-rays, cis-dichlorodiammine platinum II or N-methyl-9-hydroxy ellipticinium. The two mRNA increased 24 hours after the cell treatments but this enhanced transcription was a transient phenomenon, as it was no longer observed after 96 hours. No significant DNA amplification was detectable in the treated cells.

• OSTI ID: 5592581
• Journal Information: Biochemical and Biophysical Research Communications; (United States), Vol. 176:3; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTINEOPLASTIC DRUGS
GENETIC EFFECTS
GLYCOSYL HYDROLASES
ENZYME ACTIVITY
MESSENGER-RNA
TRANSCRIPTION
METHYL TRANSFERASES
DNA REPAIR
GAMMA RADIATION
GENE AMPLIFICATION
HEPATOMAS
HYBRIDIZATION
RATS
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ANIMAL CELLS
ANIMALS
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DISEASES
DRUGS
ELECTROMAGNETIC RADIATION
ENZYMES
HYDROLASES
IONIZING RADIATIONS
MAMMALS
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATIONS
RECOVERY
REPAIR
RNA
RODENTS
TRANSFERASES
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560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture