An autoradiographic map of (3H)diprenorphine binding in rat brain: effects of social interaction

Panksepp, J; Bishop, P

doi:10.1016/0361-9230(81)90038-1

Title: An autoradiographic map of (3H)diprenorphine binding in rat brain: effects of social interaction

Full Record
Other Related Research

Abstract

(3H)Diprenorphine binding was analyzed autoradiographically in the brains of 33 day old rat pups. A photographic atlas of diprenorphine binding in the coronal plane is provided to highlight the dispersion of opioid receptor systems through the brain. To determine whether brain opioid release may be induced by social interactions, half the animals were sacrificed following a 30 min period of social interaction while the other half were sacrificed following 30 min of social isolation. Opioid binding was higher in isolate-tested animals than socially-tested ones, suggesting that social interaction may promote endogenous brain opioid release.

Authors:: Panksepp, J; Bishop, P

Publication Date:: Thu Oct 01 00:00:00 EDT 1981

Research Org.:: Department of Psychology, Bowling Green State University, OH

OSTI Identifier:: 5589449

Resource Type:: Journal Article

Journal Name:: Brain Res. Bull.; (United States)

Additional Journal Information:: Journal Volume: 7:4

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; BRAIN; AUTORADIOGRAPHY; TRITIUM COMPOUNDS; ISOTOPE APPLICATIONS; BEHAVIOR; BIOCHEMICAL REACTION KINETICS; BIOCHEMISTRY; RATS; RECEPTORS; SOCIOLOGY; ANIMALS; BODY; CENTRAL NERVOUS SYSTEM; CHEMISTRY; KINETICS; LABELLED COMPOUNDS; MAMMALS; NERVOUS SYSTEM; ORGANS; REACTION KINETICS; RODENTS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques; 550101 - Behavioral Biology- Tracer Techniques

Citation Formats


                    Panksepp, J, and Bishop, P. An autoradiographic map of (3H)diprenorphine binding in rat brain: effects of social interaction.  United States: N. p., 1981. 
        Web.  doi:10.1016/0361-9230(81)90038-1.

Copy to clipboard


                    Panksepp, J, & Bishop, P. An autoradiographic map of (3H)diprenorphine binding in rat brain: effects of social interaction.  United States.  https://doi.org/10.1016/0361-9230(81)90038-1

Copy to clipboard


                    Panksepp, J, and Bishop, P. 1981.  
        "An autoradiographic map of (3H)diprenorphine binding in rat brain: effects of social interaction".  United States.  https://doi.org/10.1016/0361-9230(81)90038-1.

Copy to clipboard


                    
@article{osti_5589449,

  title        = {An autoradiographic map of (3H)diprenorphine binding in rat brain: effects of social interaction},

  author       = {Panksepp, J and Bishop, P},

  abstractNote = {(3H)Diprenorphine binding was analyzed autoradiographically in the brains of 33 day old rat pups. A photographic atlas of diprenorphine binding in the coronal plane is provided to highlight the dispersion of opioid receptor systems through the brain. To determine whether brain opioid release may be induced by social interactions, half the animals were sacrificed following a 30 min period of social interaction while the other half were sacrificed following 30 min of social isolation. Opioid binding was higher in isolate-tested animals than socially-tested ones, suggesting that social interaction may promote endogenous brain opioid release.},

  doi          = {10.1016/0361-9230(81)90038-1},

  url          = {https://www.osti.gov/biblio/5589449},
  journal      = {Brain Res. Bull.; (United States)},
number       = ,

  volume       = 7:4,

  place        = {United States},

  year         = {Thu Oct 01 00:00:00 EDT 1981},

  month        = {Thu Oct 01 00:00:00 EDT 1981}

}

Copy to clipboard

Journal Article:

https://doi.org/10.1016/0361-9230(81)90038-1

Other availability

Find in Google Scholar

Search WorldCat to find libraries that may hold this journal

Save / Share:

Export Metadata

Save to My Library

Similar records in OSTI.GOV collections:

An autoradiographic analysis of cholinergic receptors in mouse brain after chronic nicotine treatment

Journal Article Pauly, J; Marks, M; Gross, S; ... - Journal of Pharmacology and Experimental Therapeutics; (United States)

Quantitative autoradiographic procedures were used to examine the effects of chronic nicotine infusion on the number of central nervous system nicotinic cholinergic receptors. Female DBA mice were implanted with jugular cannulas and infused with saline or various doses of nicotine (0.25, 0.5, 1.0 or 2.0 mg/kg/hr) for 10 days. The animals were then sacrificed and the brains were removed and frozen in isopentane. Cryostat sections were collected and prepared for autoradiographic procedures as previously described. Nicotinic cholinergic receptors were labeled with L-(3H)nicotine or alpha-(125I)bungarotoxin; (3H)quinuclidinyl benzilate was used to measure muscarinic cholinergic receptor binding. Chronic nicotine infusion increased the numbermore »« less
Autoradiographic analysis of the in vivo distribution of 3H-imipramine and 3H-desipramine in brain: Comparison to in vitro binding patterns

Journal Article Duncan, G; Paul, I; Fassberg, J; ... - Pharmacology, Biochemistry and Behavior; (United States)

Using high resolution autoradiographic techniques, the distribution of radioactivity in forebrain and brainstem was assessed after 4 injection of 3H-impramine or 3H-desipramine. Results were compared with regional binding of the drugs to brain sections in vitro. Similar topographic binding of 3H-imipramine and 3H-desipramine was observed in vitro among brain regions, except in the paraventricular nucleus of the hypothalamus and locus coeruleus, where binding was greater for 3H-desipramine. For both 3H-desipramine and 3H-imipramine, some brain regions that exhibited high binding in vitro also showed high accumulation after in vivo injection. However, certain regions that contained high densities of binding sites formore »« less
https://doi.org/10.1016/0091-3057(91)90024-V
Evaluation of radioiodinated C6-O- and N-iodoallyl analogues of diprenorphine as ligands for cerebral opioid receptors

Journal Article Lever, J; Scheffel, U; Stathis, M - Journal of Nuclear Medicine

Analogues of diprenorphine (DPN) having C6-O-iodoallyl (O-IA-DPN) and N-iodoallyl (N-IA-DPN) substituents can be I-125 labeled in good yield with high specific activity by radioiododestannylation. When tested in vitro against [H-3]-DPN in rat brain membranes, the apparent affinity (Ki) of O-IA-DPN (1.35 nM) proved 17-fold stronger than that of N-IA-DPN (23.4 nM). Against selective [H-3]-ligands, O-IA-DPN showed high apparent affinities for {mu}(1.9 nM), {gamma}(1.1 nM) and {kappa}(0.9 nM) sites. Consistent with the low apparent affinity in vitro, [I-125]-N-IA- DPN did not allow localization of cerebral opioid receptors after i.v. administration to mice. By contrast, [I-125]-O-IA-DPN exhibited a regional brain distribution whichmore »« less
Endogenous opioid peptides as neurotransmitters in the rat hippocampus

Book Neumaier, J

The role of endogenous opioid peptides as neurotransmitters in the rat hippocampus was investigated by using extracellular recording and radioligand binding techniques in the hippocampal slice preparation. Synaptic conductances from endogenously released opioid peptides have been difficult to detect. This problem was approach by designing a novel assay of opioid peptide release, in which release was detected by measuring binding competition between endogenous opioids and added radioligand. Membrane depolarization displaced ({sup 3}H)-diprenorphine binding in a transient, calcium-dependent, and peptidase-sensitive manner. Autoradiographic localization of the sites of ({sup 3}H)-diprenorphine binding displacement showed that significant opioid peptide release and receptor occupancy occurredmore »« less
Autoradiographical detection of cholecystokinin-A receptors in primate brain using sup 125 I-Bolton Hunter CCK-8 and 3H-MK-329

Journal Article Hill, D; Shaw, T; Graham, W; ... - Journal of Neuroscience; (USA)

In vitro autoradiography was performed in order to visualize cholecystokinin-A (CCK-A) receptors in sections of Cynomolgus monkey brain. CCK-A receptors were defined as those which displayed high affinity for the selective non-peptide antagonist MK-329 (L-364,718) and were detected in several regions by selective inhibition of 125I-Bolton Hunter CCK using MK-329 or direct labeling with 3H-MK-329. In the caudal medulla, high densities of CCK-A sites were present in the nucleus tractus solitarius, especially the caudal and medial aspects, and also the dorsal motor nucleus of the vagus. CCK-A sites were localized to a number of hypothalamic nuclei such as the supraopticmore »« less

Similar Records