T suppressor cells are required for the maintenance of the antigen-induced B-cell unresponsive state in humans

Journal Article · Fri Apr 01 00:00:00 EST 1983 · J. Clin. Immunol.; (United States)

DOI:https://doi.org/10.1007/BF00915488· OSTI ID:5586867

Benveniste, E; Stevens, R H

Tetanus toxoid immunization of humans generates circulating B cells which secrete IgG anti-tetanus toxoid antibodies (IgG-Tet) when stimulated in vitro with T cells and pokeweed mitogen (PWM). A unique property of these cells is the inhibition of maturation into antibody-secreting plasma cells following a 1-hr in vitro pulse with tetanus toxoid. Studies were undertaken to determine if different T-cell subsets could modulate the in vitro generated B-cell unresponsive state. The addition of OKT4+/OKT8- cells to antigen-treated B cells resulted in a partial reversal of the antigen-induced inhibition of IgG-Tet synthesis. The addition of OKT4-/OKT8+ cells to the treated B cells caused a suppression of IgG-Tet synthesis comparable to that seen in cultures containing unfractionated T cells. These results indicate that (1) the B-cell unresponsive state generated by antigen treatment is not absolute, (2) the degree of B-cell unresponsiveness results from a balance of suppressor and helper signals, and (3) T-suppressor cells need to be present to induce and maintain the B-cell unresponsive state.

• Research Organization: Department of Microbiology and Immunology, University of California, Los Angeles, School of Medicine
• OSTI ID: 5586867
• Journal Information: J. Clin. Immunol.; (United States), Vol. 3:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
LYMPHOCYTES
IMMUNE REACTIONS
IMMUNITY
INOCULATION
MONOCLONAL ANTIBODIES
TETANUS
TOXINS
VACCINES
ANIMAL CELLS
ANTIBODIES
ANTIGENS
BACTERIAL DISEASES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DISEASES
INFECTIOUS DISEASES
LEUKOCYTES
MATERIALS
SOMATIC CELLS
TOXIC MATERIALS
550900* - Pathology