Rat submaxillary gland contains predominantly P-type tachykinin binding sites
The specific binding of the /sup 125/I-Bolton-Hunter labeled tachykinins substance K (BHSK), eledoisin (BHE), and substance P (BHSP) was examined in crude membrane suspensions and by autoradiography in rat submaxillary gland. All three ligands at 0.1 nM concentrations exhibited binding that was inhibited by tachykinins in a potency rank order of substance P greater than physalaemin greater than substance K greater than eledoisin greater than kassinin greater than neuromedin K with slope factors essentially equal to unity. All tachykinins were 5 to 10 times more potent in inhibiting BHSK and BHE binding compared to BHSP binding. Autoradiographic visualization of BHSK and BHSP binding sites in the gland revealed extensive labeling of mucous and serous acini. The intensity of labeling was much less for BHSK than for BHSP. The results indicate that the rat submaxillary gland contains predominantly P-type tachykinin binding sites.
- Research Organization:
- Section on Biochemical Pharmacology, NHLBI, Bethesda, MD
- OSTI ID:
- 5578781
- Journal Information:
- Peptides (Fayetteville, N.Y.); (United States), Vol. 6
- Country of Publication:
- United States
- Language:
- English
Similar Records
New type of tachykinin binding site in the rat brain characterized by specific binding of a labeled eledoisin derivative
( sup 125 I)-Bolton-Hunter scyliorhinin II: A novel, selective radioligand for the tachykinin NK3 receptor in rat brain
Related Subjects
PEPTIDES
BIOCHEMICAL REACTION KINETICS
RECEPTORS
AUTORADIOGRAPHY
CELL MEMBRANES
COMPARATIVE EVALUATIONS
GLANDS
IODINE 125
LIGANDS
PROTEINS
RATS
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
RADIOISOTOPES
REACTION KINETICS
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques