Cell cycle analysis of cultured mammalian cells after exposure to 4,5',8-trimethylpsoralen and long-wave ultraviolet light
Cell cycle analysis was used to study the the effect of 4,5'8-trimethylpsoralen (TMP) and long-wave ultraviolet light (UV-A) on cultured mammalian cells. DNA distribution patterns were measured for murine melanoma cells (a cloned line of Cloudman S91) and a strain of diploid human skin fibroblasts (CRL 1295) using both a microfluorimetry procedure and flow cytometry. The untreated cells and those receiving TMP along and UV-A alone had identical DNA content as assessed at several posttreatment intervals (0-72 hr). The majority of cells in control groups contained a G1 DNA content, whereas exposure to TMP (2 x 10(-7) M) plus UV-A (1 Joule/cm2) led to the accumulation of cells in the G2 phase. These observations were similar for each cell type and both analytical techniques were in excellent agreement. The finding that psoralen plus UV-A induces a phase-specific G2 blockade in cultured cells has important implications for understanding the mechanisms which account for enhanced pigmentation and suppression of cellular proliferation following exposure to these agents in vivo.
- OSTI ID:
- 5577048
- Journal Information:
- J. Invest. Dermatol.; (United States), Vol. 76:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
FIBROBLASTS
BIOLOGICAL RADIATION EFFECTS
MELANOMAS
PSORALEN
RADIOSENSITIVITY EFFECTS
BIOLOGICAL PATHWAYS
CELL CULTURES
CELL CYCLE
CELL FLOW SYSTEMS
CELL PROLIFERATION
DNA
MELANIN
TUMOR CELLS
ULTRAVIOLET RADIATION
ANIMAL CELLS
ANTICOAGULANTS
BIOLOGICAL EFFECTS
CONNECTIVE TISSUE CELLS
COUMARINS
DISEASES
DRUGS
ELECTROMAGNETIC RADIATION
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PIGMENTS
RADIATION EFFECTS
RADIATIONS
SOMATIC CELLS
560121* - Radiation Effects on Cells- External Source- (-1987)
550300 - Cytology