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Title: Host cell reactivation of sunlamp-exposed adenovirus in fibroblasts from patients with Bloom's syndrome, ataxia telangiectasia, and Huntington's disease

Abstract

In this study, a sensitive host cell reactivation (HCR) technique was used to examine the repair capacity for DNA damaged by sunlamp exposure in fibroblast strains derived from 5 normal individuals and 8 patients representing three different diseases associated with DNA repair deficiencies. Adenovirus type 2 (Ad 2) was exposed to radiation from a GE 275 W sunlamp and subsequently used to infect fibroblast monolayers. At 48 hr after infection, cells were scored for the presence of viral structural antigens (Vag) using indirect immunofluorescent staining. Previous reports using this technique showed a substantial reduction in the HCR of sunlamp-exposed Ad 2 for infection of excision repair deficient fibroblasts from patients with xeroderma pigmentosum. In contrast, the HCR of Vag synthesis for sunlamp-exposed Ad 2 was in the normal range for the three ataxia telangiectasia, three Bloom's syndrome, and two Huntington's disease fibroblasts strains.

Authors:
 [1]
  1. McMaster Univ., Hamilton, Ontario (Canada)
Publication Date:
OSTI Identifier:
5574269
Resource Type:
Journal Article
Journal Name:
Environmental and Molecular Mutagenesis; (USA)
Additional Journal Information:
Journal Volume: 17:2; Journal ID: ISSN 0893-6692
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ADENOVIRUS; BIOLOGICAL RADIATION EFFECTS; FIBROBLASTS; DNA REPAIR; NERVOUS SYSTEM DISEASES; TELANGIECTASIS; ULTRAVIOLET RADIATION; XERODERMA PIGMENTOSUM; ANIMAL CELLS; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; CONNECTIVE TISSUE CELLS; DISEASES; ELECTROMAGNETIC RADIATION; MICROORGANISMS; ONCOGENIC VIRUSES; PARASITES; RADIATION EFFECTS; RADIATIONS; RECOVERY; REPAIR; SKIN DISEASES; SOMATIC CELLS; VASCULAR DISEASES; VIRUSES; 560120* - Radiation Effects on Biochemicals, Cells, & Tissue Culture; 560130 - Radiation Effects on Microorganisms

Citation Formats

Rainbow, A J. Host cell reactivation of sunlamp-exposed adenovirus in fibroblasts from patients with Bloom's syndrome, ataxia telangiectasia, and Huntington's disease. United States: N. p., 1991. Web. doi:10.1002/em.2850170206.
Rainbow, A J. Host cell reactivation of sunlamp-exposed adenovirus in fibroblasts from patients with Bloom's syndrome, ataxia telangiectasia, and Huntington's disease. United States. https://doi.org/10.1002/em.2850170206
Rainbow, A J. 1991. "Host cell reactivation of sunlamp-exposed adenovirus in fibroblasts from patients with Bloom's syndrome, ataxia telangiectasia, and Huntington's disease". United States. https://doi.org/10.1002/em.2850170206.
@article{osti_5574269,
title = {Host cell reactivation of sunlamp-exposed adenovirus in fibroblasts from patients with Bloom's syndrome, ataxia telangiectasia, and Huntington's disease},
author = {Rainbow, A J},
abstractNote = {In this study, a sensitive host cell reactivation (HCR) technique was used to examine the repair capacity for DNA damaged by sunlamp exposure in fibroblast strains derived from 5 normal individuals and 8 patients representing three different diseases associated with DNA repair deficiencies. Adenovirus type 2 (Ad 2) was exposed to radiation from a GE 275 W sunlamp and subsequently used to infect fibroblast monolayers. At 48 hr after infection, cells were scored for the presence of viral structural antigens (Vag) using indirect immunofluorescent staining. Previous reports using this technique showed a substantial reduction in the HCR of sunlamp-exposed Ad 2 for infection of excision repair deficient fibroblasts from patients with xeroderma pigmentosum. In contrast, the HCR of Vag synthesis for sunlamp-exposed Ad 2 was in the normal range for the three ataxia telangiectasia, three Bloom's syndrome, and two Huntington's disease fibroblasts strains.},
doi = {10.1002/em.2850170206},
url = {https://www.osti.gov/biblio/5574269}, journal = {Environmental and Molecular Mutagenesis; (USA)},
issn = {0893-6692},
number = ,
volume = 17:2,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 1991},
month = {Tue Jan 01 00:00:00 EST 1991}
}