Rabbit uterine epithelial cells: Co-culture with spermatozoa
A primary culture of rabbit uterine epithelial cells was established and their effects on sperm function were examined in vitro. Epithelial cells were isolated from uteri of estrous rabbits and cultured on floating collagen gels in phenol red-free medium supplemented with 5% fetal bovine serum. Light microscopy and keratin staining showed that the epithelial cell population established in culture had morphological characteristics similar to that seen in the intact endometrium. Cells were cultured with {sup 3}H-leucine and uptake of label by cells and its incorporation into cellular and secretory proteins determined. When compared to cells cultured for 24-48 h, incorporation of label into cellular protein was lower at 72-96 h, but secretion increased. Estradiol 17-{beta} did not affect label uptake or incorporation, but did enhance proliferation of cells as judged by total DNA content of the cell population. Analysis of proteins in media by sodium dodecyl sulfate polyacrylamide gel electrophoresis and fluorography suggested that epithelial and stromal cells synthesis proteins that may be secretory in nature during 72-96 h culture. Twenty-nine to thirty-one h after initiation of epithelial cultures, 1-2 {times} 10{sup 6} sperm were co-incubated with cells and sperm viability, motility, loss of acrosome and fertilizing ability determined.
- Research Organization:
- Massachusetts Univ., Amherst, MA (USA)
- OSTI ID:
- 5564075
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANIMAL CELLS
CELL PROLIFERATION
PROTEINS
BIOSYNTHESIS
SPERMATOZOA
VIABILITY
CELL CULTURES
CULTURE MEDIA
DNA
ELECTROPHORESIS
EPITHELIUM
ESTRADIOL
FLUOROSCOPY
LEUCINE
MORPHOLOGY
RABBITS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UTERUS
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
BIOMEDICAL RADIOGRAPHY
BODY
CARBOXYLIC ACIDS
DIAGNOSTIC TECHNIQUES
ESTRANES
ESTROGENS
FEMALE GENITALS
GAMETES
GERM CELLS
HORMONES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
MEDICINE
NUCLEAR MEDICINE
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RADIOLOGY
STEROID HORMONES
STEROIDS
SYNTHESIS
TISSUES
VERTEBRATES
550301* - Cytology- Tracer Techniques