Mechanism of depression of the spinal pain syndrome by serotonin derivatives

Journal Article · Mon Sep 01 00:00:00 EDT 1986 · Bull. Exp. Biol. Med. (Engl. Transl.); (United States)

DOI:https://doi.org/10.1007/BF00834397· OSTI ID:5564016

Kryzhanovskii, G N; Lutsenko, N G; Grafova, V N; Danilova, E I; Lutsenko, V K; Gordeev, E N; Kalacheva, N A; Suvorov, N N

The authors compare the ability of 5-HT derivatives to stimulate adenylate cyclase in the nervous system with their action on the intensity of the spinal pain syndrome (SPS), induced by the creation of a generator of pathologically enhanced excitation in the lumbar segments of the spinal cord of rats. An SPS was induced in rats by applying the sodium salt of benzylpenicillin to the dorsal surface of the lumbar segments of the spinal cord. The effect of 5-HT derivatives on activity of serotonin-sensitive adenylate cyclase was estimated from the change in cAMP concentration in the synaptosomes. During the experiments, the disintegrated synaptosomes were removed by centrifugation after which the cAMP concentration in the supernatant was determined by means of a kit of reagents for radioligand determination of cAMP. Radioactivity was measured on a scintillation spectrometer.

• Research Organization: Research Institute of General Pathology and Pathological Physiology, Moscow, USSR
• OSTI ID: 5564016
• Journal Information: Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Vol. 101:4; Other Information: Translated from Byulleten' Ehksperimental'noj Biologii i Meditsiny; 101: No. 4, 399-401(Apr 1986)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AMP
RADIOASSAY
NERVE CELLS
SEROTONIN
BIOLOGICAL EFFECTS
METABOLISM
SPINAL CORD
PAIN
BIOCHEMISTRY
CYCLASES
ENZYME ACTIVITY
INHIBITION
QUANTITY RATIO
RATS
RECEPTORS
SCINTILLATION COUNTING
AMINES
ANIMAL CELLS
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZOLES
CENTRAL NERVOUS SYSTEM
CHEMISTRY
COUNTING TECHNIQUES
DRUGS
ENZYMES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INDOLES
LYASES
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
NEUROREGULATORS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PYRROLES
RADIOPROTECTIVE SUBSTANCES
RODENTS
SOMATIC CELLS
SYMPATHOMIMETICS
SYMPTOMS
TRYPTAMINES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
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