Chick neural retina adhesion and survival molecule is a retinol-binding protein

Journal Article · Wed Jan 01 00:00:00 EST 1986 · J. Cell Biol.; (United States)

DOI:https://doi.org/10.1083/jcb.102.6.2295· OSTI ID:5563908

Schubert, D; LaCorbiere, M; Esch, F

A 20,000-D protein called purpurin has recently been isolated from the growth-conditioned medium of cultured embryonic chick neural retina cells. Purpurin is a constituent of adherons and promotes cell-adheron adhesion by interacting with a cell surface heparan sulfate proteoglycan. It also prolongs the survival of cultured neural retina cells. This paper shows that purpurin is a secretory protein that has sequence homology with a human protein synthesized in the liver that transports retinol in the blood, the serum retinol-binding protein (RBP). Purpurin binds (/sup 3/H)retinol, and both purpurin and chick serum RBP stimulate the adhesion of neural retina cells, although the serum protein is less active than purpurin. Purpurin and the serum RBP are, however, different molecules, for the serum protein is approx.3.000 D larger than purpurin and has different silver-staining characteristics. Finally, purpurin supports the survival of dissociated ciliary ganglion cells, indicating that RBPs can act as ciliary neurotrophic factors.

• Research Organization: Salk Institute for Biological Studies, San Diego, CA
• OSTI ID: 5563908
• Journal Information: J. Cell Biol.; (United States), Vol. 102
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
VITAMIN A
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HEPARIN
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NERVE CELLS
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TRITIUM COMPOUNDS
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ANIMAL CELLS
ANTICOAGULANTS
CARBOHYDRATES
DRUGS
HEMATOLOGIC AGENTS
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POLYSACCHARIDES
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SACCHARIDES
SOMATIC CELLS
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