Rhizobium meliloti produces a family of sulfated lipo-oligosaccharides exhibiting different degrees of plant host specificity

Journal Article · Wed Jan 01 00:00:00 EST 1992 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.89.1.192· OSTI ID:5560583

Schultze, M; Kondorosi, E; Quiclet-Sire, B; Gero, S D ^[1]; Virelizier, H ^[2]; Glushka, J N ^[3]; Endre, G; Kondorosi, A ^[4]

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1. Centre National de la Recherche Scientifique, Gif-sur-Yvette (France)
2. Centre d'Etudes Nucleaires, Gif-sur-Yvette (France)
3. Univ. of Georgia, Athens (United States)
4. Centre National de la Recherche Scientifique, Gif-sur-Yvette (France) Inst. of Genetics, Szeged (Hungary)

The authors have shown that a Rhizobium meliloti strain over expressing nodulation genes excreted high amounts of a family of N-acylated and 6-O-sulfated N-acetyl-{beta}-1,4-D-glucosamine penta-, tetra-, and trisaccharide Nod factors. Either a C{sub 16:2} or a C{sub 16:3} acyl chain is attached to the nonreducing end subunit, whereas the sulfate group is bound to the reducing glucosamine. In the root hair deformation assay the pentasaccharides show similar activities on the host plants Medicago sativa and Melilotus albus and on the non-host plant Vicia sativa at a dilution of up to 0.01-0.001 {mu}M, in contrast to NodRm-1, which displays a much higher specific activity for Medicago and melilotus than for Vicia. The active concentration range of the pentasaccharides is more narrow on medicago than on Melilotus and Vicia. In addition to root hair deformation, the different Nod factors were shown to induce nodule formation on M. sativa. They suggest that the production of a series of active signal molecules with different degrees of specificity might be important in controlling the symbiosis of R. meliloti with several different host plants or under different environmental conditions.

• DOE Contract Number: FG09-87ER13810
• OSTI ID: 5560583
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 89:1; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
LIPOPOLYSACCHARIDES
NUCLEAR MAGNETIC RESONANCE
RHIZOBIUM
SYMBIOSIS
CARBON 13
GLUCOSAMINE
HOST
PLANT GROWTH
PROTONS
ROOTS
AMINES
BACTERIA
BARYONS
CARBOHYDRATES
CARBON ISOTOPES
ELEMENTARY PARTICLES
EVEN-ODD NUCLEI
FERMIONS
GROWTH
HADRONS
HEXOSAMINES
HEXOSES
ISOTOPES
LIGHT NUCLEI
LIPIDS
MAGNETIC RESONANCE
MICROORGANISMS
MONOSACCHARIDES
NUCLEI
NUCLEONS
ORGANIC COMPOUNDS
POLYSACCHARIDES
RESONANCE
SACCHARIDES
STABLE ISOTOPES
550200* - Biochemistry